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Merge pull request #6509 from paulzierep/update-decontam
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Add description to decontam
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bgruening authored Mar 9, 2025
2 parents 0b67127 + 1c287d4 commit 8eca6c0
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13 changes: 13 additions & 0 deletions tools/decontam/decontam.xml
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<tool id="decontam" name="Decontam" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
<description>Identification and removal of contaminants</description>
<macros>
<import>macros.xml</import>
</macros>
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Currently, only the negative control sample method is implemented in this
wrapper.
**Method**
To identify contaminants decontam requires a defined set of “negative control” samples in
which sequencing was performed on blanks without any biological sample added.
Extraction controls are preferred, and in amplicon sequencing the negative controls
should also be carried through the PCR step, as each step in the workflow
has the potential to introduce new contaminants.
Decontam then uses Fisher’s exact tests to detect weather a feature is a contaminant or not,
by comparing true samples to ngeative controls. For more details on the detection method see
the publication of decontam.
**Output**
- If a phyloseq object is provided as input, the output will be a phyloseq
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