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PD-2559andPD-2558: UMI filtering and cell barcode correction #1263

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merged 10 commits into from
Apr 25, 2024

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aawdeh
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@aawdeh aawdeh commented Apr 12, 2024

Combined two tickets into one to test out changes and merge changes combined

Tackling both UMI filtering and cell barcode correction

Changed/Added starsolo parameters:

  • soloCBmatchWLtype is now set to1MM_multi
  • soloUMIdedup is now set to 1MM_CR
  • soloUMIfiltering is now set to MultiGeneUMI_CR

Smart tests failed on: dev_10k_pbmc_downsampled, pbmc_human_v3, mouse_v2_snRNA_example and snRNA_mouse_v2_snRNA_example

Notebook to compare failed smart tests: https://app.terra.bio/#workspaces/warp-pipelines/Multiome-test-workspace/analysis/launch/PD2559_PD2558_umi_filtering_and_cell_barcode_correction.ipynb?mode=edit

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Checklist

If you can answer "yes" to the following items, please add a checkmark next to the appropriate checklist item(s) and notify our WARP documentation team by tagging either @ekiernan or @kayleemathews in a comment on this PR.

  • Did you add inputs, outputs, or tasks to a workflow?
  • Did you modify, delete or move: file paths, file names, input names, output names, or task names?
  • If you made a changelog update, did you update the pipeline version number?

…Idedup:1MM_CR and soloUMIfiltering:MultiGeneUMI_CR
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Remember to squash merge!

@aawdeh aawdeh changed the title PD-2559 and PD-2558 PD-2559andPD-2558: UMI filtering and cell barcode correction Apr 12, 2024
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Remember to squash merge!

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Remember to squash merge!

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Validation Results:

Comparing versions and changelogs for pipelines that differ from the versions on 'origin/develop':
All WDLs and changelog files appear to be valid for this release.

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@kevinpalis kevinpalis left a comment

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LGTM

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@khajoue2 khajoue2 left a comment

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lgtm

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Remember to squash merge!

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Validation Results:

Comparing versions and changelogs for pipelines that differ from the versions on 'origin/develop':
All WDLs and changelog files appear to be valid for this release.

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Remember to squash merge!

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Validation Results:

Comparing versions and changelogs for pipelines that differ from the versions on 'origin/develop':
All WDLs and changelog files appear to be valid for this release.

@ekiernan
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retest this please

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@ekiernan
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retest this please

@aawdeh aawdeh merged commit d7aef8f into develop Apr 25, 2024
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@aawdeh aawdeh deleted the aa-chimeric_and_barcode branch April 25, 2024 14:05
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4 participants