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Add new BAM example for full PG entry #245

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73 changes: 73 additions & 0 deletions noodles-bam/examples/bam_add_tag_to_header.rs
Original file line number Diff line number Diff line change
@@ -0,0 +1,73 @@
//! Add a PG tag to the SAM header of a BAM file.
//!
//! This is a demonstration of how to construct a full PG entry.
//!
//! Verify the output by piping to `samtools view --with-header`.

use std::{env, io};

use noodles_bam as bam;
use noodles_sam::header::record::value::{
map::{Program, Tag},
Map,
};
use noodles_sam::Header;

fn add_pg(mut header: Header) -> Header {
const NAME: &str = "bam_add_tag_to_header";
const VERSION: &str = env!("CARGO_PKG_VERSION");

// standard PG tags PN, VN, PP, CL
let pn = match Tag::try_from([b'P', b'N']) {
Ok(Tag::Other(tag)) => tag,
_ => unreachable!(),
};
let vn = match Tag::try_from([b'V', b'N']) {
Ok(Tag::Other(tag)) => tag,
_ => unreachable!(),
};
let pp = match Tag::try_from([b'P', b'P']) {
Ok(Tag::Other(tag)) => tag,
_ => unreachable!(),
};
let cl = match Tag::try_from([b'C', b'L']) {
Ok(Tag::Other(tag)) => tag,
_ => unreachable!(),
};
// the command-line to insert into the CL tag
let cmd_str = env::args().collect::<Vec<_>>().join(" ");
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Owner

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Extra care needs to be taken for arguments containing tab characters.


let program = Map::<Program>::builder().insert(pn, NAME);

let program = if let Some(last_pg) = header.programs().keys().last() {
program.insert(pp, last_pg.clone())
} else {
program
};
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Header records are not guaranteed to be ordered by recency. This would be incorrect for, e.g.,

@PG	ID:pg1	PP:pg0
@PG	ID:pg0

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Ah, so this is what you mean by program-chaining--you want to parse the order during Program construction?


let program = program
.insert(vn, VERSION)
.insert(cl, cmd_str)
.build()
.unwrap();
header.programs_mut().insert(NAME.into(), program);

header
}

fn main() -> io::Result<()> {
let src = env::args().nth(1).expect("missing src");

let mut reader = bam::io::reader::Builder.build_from_path(src)?;

let header = add_pg(reader.read_header()?);

let stdout = io::stdout().lock();
let mut writer = bam::io::Writer::new(stdout);

writer.write_header(&header)?;

io::copy(reader.get_mut(), writer.get_mut())?;

Ok(())
}