Add this suggestion to a batch that can be applied as a single commit.
This suggestion is invalid because no changes were made to the code.
Suggestions cannot be applied while the pull request is closed.
Suggestions cannot be applied while viewing a subset of changes.
Only one suggestion per line can be applied in a batch.
Add this suggestion to a batch that can be applied as a single commit.
Applying suggestions on deleted lines is not supported.
You must change the existing code in this line in order to create a valid suggestion.
Outdated suggestions cannot be applied.
This suggestion has been applied or marked resolved.
Suggestions cannot be applied from pending reviews.
Suggestions cannot be applied on multi-line comments.
Suggestions cannot be applied while the pull request is queued to merge.
Suggestion cannot be applied right now. Please check back later.
I got the same error when the BAM file had zero coverage in al least one (but not all) regions described in the BED file.
I solved by editing the samtools command by providing an additional "-a" option
my $extractcov = "samtools depth -r $chr:$start-$stop -a -a -q 0 -Q 0 $bam | awk '{sum+=$3} END {if (NR > 0) print sum/NR; else print 0;}'";
(see the my edit "-a" after the first "-a")
In this way samtools produces the expected output with 0 coverage in every position.
Versions