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# rnaseq_demo RNAseq Demo # Author: Kate Mortensen # Last Update: 04-21-2021 # The purpose of this repo is to provide a demo for RNA seq analysis and differential expression. # The necessary libraries and tools are defined in the Singularity rna.def recipe file. # The necessary data preprocessing commands are defined in the rnaseq.sh script. # The diff_expression.R script produces output for analysis of differential expression. # TODO # Create the following directories in the rnaseq_demo directory: sequences, genome, results, counts, alignment_results. # The user will have to manually download the sequences listed in the sequences.txt file into the sequences directory. # Once this is done, the user should be able to execute the rnaseq.sh script and diff_expression.R script without an issue.
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