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A simple RNA-Seq pipeline for transcript quantification using DESeq2 and edgeR.

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This section aims to reanalyze the data retrived from a publication by Griffith et al. (2015).

TODO:

  • retrieve and extract data: reference genome (.fasta), reads (.fastq), annotation file (.gff)
  • generate reference index (.idx)
  • align reads to reference (.sam)
  • convert SAM to BAM (.bam)
  • generate index for BAM files
  • OPTIONAL: convevrt BAM to bigWig for visualization
  • count overlapping alignments (count matrix)
  • standardize count matrix
  • run statistical model for analyzing counts
  • produce heat map from standardized counts

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A simple RNA-Seq pipeline for transcript quantification using DESeq2 and edgeR.

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