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# DBGDE | ||
Using pre-made software trying to implement Mutual software algorithm to get differential expression. | ||
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We have: | ||
- One implementation trying to get GFA2 working at: https://github.com/Omig12/Mutual-pepino | ||
- The one I just did is GFA1 with GFApy working and beign read by Bandage. Used segment for both fragment and the segment, the Segment appears first follow by the kmer subsegments. | ||
``` | ||
Format of GFA1 Segment is: S id_of_original_segment contig. | ||
``` | ||
``` | ||
Format of GFA1 subSegment is: S id_of_original_segment:subsegmentid:(A:count,B:count) kmer | ||
``` | ||
Where id_of_original_segment is the same as first segment, subsegmentid is this kmer id, a tuple with the count of all the times that kmer appears in the organism. | ||
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An example to run the dbg.py is: | ||
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``` | ||
python dbg.py -k <kmer_size> -A <Organism A file(s)> -B <Organism B file(s)> | ||
``` |