-
Notifications
You must be signed in to change notification settings - Fork 0
NCI-RBL/CLIP_Analysis_Soyeong
Folders and files
Name | Name | Last commit message | Last commit date | |
---|---|---|---|---|
Repository files navigation
Analysis Script is designed to run Single CLIP sample at a time from the CLIP_Pipeline. The script will generate two Report files per sample: SampleName: Structure Analysis.html SampleName: LINE/SINE Distance.html 1. Run CLIP_Pipeline 2. Run Random CLIP data : RandomCLIP_V2.sh - Arg1 - Number of permutation - Arg2 - window size - Arg3 - minimum average chuck basepair probability - Arg4 - minimum Max chuck basepair probability - Arg5 - number of CPUs for individual tasks - Arg6 - batch number - Arg7 - CLIPtype - Arg8 - RunMode - Arg9 - TrasncLoc - 3. Place folder “CLIPoutput/CLIP_Structure_Analsysis” into Pipeline output Folder 4. Edit CLIP_Analysis.sh script for settings related to output - Note: Generating MotifAnalysis Figures and number of permutations for LINE/SINE distance will greatly increase run time - LINE/SINE Distance analysis should only have to be run once per sample as it generates all permutations in a single output ## Set Analysis mode **CLIPtype:** Introns - Select peaks that are classified as "Protein Coding : Exons" Exons - Select peaks that are classified as "Protein Coding : Introns" Introns_LINESINETLR - Select peaks that are classified as Protein Coding : Introns and Intron peaks that are classified as "Repeat Elements" but overelap with LINE SINE or TLR **RunMode:** Genomic - Include Intronic and Exonic Sequences when finding 100nt region Upsteram and Downstream of CLIP peak Transcriptomic - Only include Exonic Sequences when finding 100nt region Upsteram and Downstream of CLIP peak. If Upstream/Downstream region crosses Intronic region, the intronic sequence is skpped and the next exon in included. **TranscLoc:** select subset of Protein Coding CLIP **window** Select the number of nts upstream and downstream of the 5’ CLIP peak in which you would like to interrogate. ## Nucleotide Content Set the sliding window size and number of clusters for the Nucleotide content Heatmap ## Local Structure Settings **meanBPP** select the minimum, average base paring probability of a 10nt chuck in the Upstream region of the CLIP peak to cluster on the Heatmap **LScluster** Select number of clusters to Run
About
No description, website, or topics provided.
Resources
Stars
Watchers
Forks
Releases
No releases published
Packages 0
No packages published