diff --git a/openthread/cmake/extensions.cmake b/openthread/cmake/extensions.cmake
index ce05442fe9..81b99c8a48 100644
--- a/openthread/cmake/extensions.cmake
+++ b/openthread/cmake/extensions.cmake
@@ -98,7 +98,7 @@ function(get_active_mbedtls_configs_from_file fileName returnMatch1List)
 endfunction()
 
 function(check_openthread_dependencies ot_lib_nrf_security_mbedtls_config_file)
-  set(nrf_security_mbedtls_config_file "${CMAKE_CURRENT_BINARY_DIR}/../nrf_security/include/${CONFIG_MBEDTLS_CFG_FILE}")
+  set(nrf_security_mbedtls_config_file "${CMAKE_CURRENT_BINARY_DIR}/../nrf_security/src/include/generated/${CONFIG_MBEDTLS_CFG_FILE}")
   get_active_mbedtls_configs_from_file(${nrf_security_mbedtls_config_file} mbedtls_conf_list)
   get_active_mbedtls_configs_from_file(${ot_lib_nrf_security_mbedtls_config_file} ot_mbedtls_conf_list)