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error with cramino #36

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HannahBenisty opened this issue Feb 22, 2025 · 1 comment
Open

error with cramino #36

HannahBenisty opened this issue Feb 22, 2025 · 1 comment

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@HannahBenisty
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HannahBenisty commented Feb 22, 2025

Hi,

I am trying to run cramino with bam files obtained from the P2Solo sequencing run and I keep getting this message. Any hint what could be happening?

cramino --threads 6 PBA84545_pass_4cb36bae_72a9ecbb_592.bam

Many thanks.

[2025-02-22T12:19:40Z ERROR cramino] Not enough alignments to calculate metrics!
thread 'main' panicked at src/main.rs:168:9:
explicit panic
note: run with RUST_BACKTRACE=1 environment variable to display a backtrace
cramino.e97903577 (END)

@wdecoster
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Could it be that the reads are not yet aligned? If so, you can add the --ubam flag.

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