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I know this is an old problem, but I belive I fixed it here: #126
devtools::install_github("rrydbirk/velocyto.R")
At least it worked on my dataset with the same error.
However, that many cells require endless computation time (trust me, the visualization will take days!). I would suggest downsampling instead to, e.g., 20-30k cells max. This could be done through sample(cellnames, 2e4). Also, be aware that Velocyto is not really made for multi-sample inputs.
fails on a very large single-cell dataset (in this case ~80k cells)
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