diff --git a/files/galaxy/tpv/tool_defaults.yml b/files/galaxy/tpv/tool_defaults.yml
index 19d3d0b3c..87e33030a 100644
--- a/files/galaxy/tpv/tool_defaults.yml
+++ b/files/galaxy/tpv/tool_defaults.yml
@@ -8,7 +8,12 @@ tools:
     mem: cores * 3.8
     gpus: 0
     env:
-      GALAXY_MEMORY_MB: "{int(mem * 1024)}" # set 5/2023 might be moved to runner or tool wrappers, related to Galaxy issue 15952
+      - name: GALAXY_MEMORY_MB
+        value: "{int(mem * 1024)}" # set 5/2023 might be moved to runner or tool wrappers, related to Galaxy issue 15952
+      - name: HDF5_USE_FILE_LOCKING
+        value: "FALSE"
+      - name: SINGULARITYENV_HDF5_USE_FILE_LOCKING
+        value: $HDF5_USE_FILE_LOCKING
     params:
       metadata_strategy: "extended"
       tmp_dir: true