diff --git a/group_vars/htcondor-secondary-submit-host.yml b/group_vars/htcondor-secondary-submit-host.yml
index fbcbc9b36..139662807 100644
--- a/group_vars/htcondor-secondary-submit-host.yml
+++ b/group_vars/htcondor-secondary-submit-host.yml
@@ -28,6 +28,17 @@ nspawn_config: |
   [Network]
   VirtualEthernet=no
 
+nspawn_galaxy_environment_file: "{{ galaxy_user.home }}/env"
+nspawn_galaxy_environment_vars: |
+  HOME={{ galaxy_root }}
+  VIRTUAL_ENV={{ galaxy_venv_dir }}
+  PATH={{ galaxy_venv_dir }}/bin:/usr/local/bin:/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/sbin
+  DOCUTILSCONFIG=''
+  PYTHONPATH={{ galaxy_server_dir }}/lib/galaxy/jobs/rules
+  {% for var in galaxy_systemd_handler_env | split %}
+  {{ var }}
+  {% endfor %}
+
 nspawn_enable: false
 nspawn_start: false
 
@@ -61,7 +72,7 @@ nspawn_ssh_authorized_keys:
   - "ssh-rsa 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 cloud@vgcn"
 nspawn_ssh_host_trust_container: yes
 
-nspawn_condor_systemd_run: "/usr/bin/systemd-run --uid={{ galaxy_user.uid }} --gid={{ galaxy_group.gid }} --pipe --quiet --machine {{ nspawn_name }}"
+nspawn_condor_systemd_run: "/usr/bin/systemd-run -p EnvironmentFile={{ nspawn_galaxy_environment_file }} --uid={{ galaxy_user.uid }} --gid={{ galaxy_group.gid }} --pipe --quiet --machine {{ nspawn_name }}"
 nspawn_condor_rm_command: "{{ nspawn_condor_systemd_run }} /usr/bin/condor_rm"
 nspawn_condor_ssh_to_job_command: "{{ nspawn_condor_systemd_run }} /usr/bin/condor_ssh_to_job"
 nspawn_condor_submit_command: "{{ nspawn_condor_systemd_run }} /usr/bin/condor_submit"
diff --git a/htcondor.yml b/htcondor.yml
index ad799d8ee..5253aacac 100644
--- a/htcondor.yml
+++ b/htcondor.yml
@@ -11,6 +11,7 @@
       changed_when: true
   vars_files:
     - mounts/mountpoints.yml
+    - secret_group_vars/all.yml
     - secret_group_vars/htcondor-secondary-submit-host.yml
   pre_tasks:
     # Because it is already disabled for sn06 and this setup is needed just
@@ -22,19 +23,19 @@
   roles:
     - kysrpex.systemd_nspawn
   post_tasks:
+    - name: Get the location of the container image.
+      ansible.builtin.shell:
+        executable: /bin/bash
+        cmd: |
+          set -o pipefail
+          machinectl image-status htcondor | grep "Path: " | awk '{$1=$1};1' | cut -d' ' -f2
+      register: nspawn_image
+      changed_when: false
+
     - name: Configure the container's sshd.
       when: nspawn_ssh and (nspawn_ssh_config is defined or nspawn_ssh_config_path is defined)
       become: true
       block:
-        - name: Get the location of the container image.
-          ansible.builtin.shell:
-            executable: /bin/bash
-            cmd: |
-              set -o pipefail
-              machinectl image-status htcondor | grep "Path: " | awk '{$1=$1};1' | cut -d' ' -f2
-          register: nspawn_image
-          changed_when: false
-
         - name: Write the sshd configuration to sshd_config.
           ansible.builtin.lineinfile:
             path: "{{ (nspawn_image.stdout, nspawn_ssh_config_path | regex_replace('(\\/*)?(.*)', '\\2')) | path_join }}"
@@ -192,6 +193,14 @@
           - "{{ nspawn_condor_ssh_to_job_command }} *"
           - "{{ nspawn_condor_submit_command }} *"
 
+    - name: Make the environment variables available to the Galaxy handlers also available to the container.
+      ansible.builtin.copy:
+        content: "{{ nspawn_galaxy_environment_vars }}"
+        dest: "{{ (nspawn_image.stdout, nspawn_galaxy_environment_file | regex_replace('(\\/*)?(.*)', '\\2')) | path_join }}"
+        owner: "{{ galaxy_user.name }}"
+        group: "{{ galaxy_group.name }}"
+        mode: "0440"
+
 - name: HTCondor cluster.
   hosts: htcondor:!sn06.galaxyproject.eu
   handlers: