diff --git a/files/galaxy/tpv/tools.yml b/files/galaxy/tpv/tools.yml
index 3cde3e8fc..de3b13ef4 100644
--- a/files/galaxy/tpv/tools.yml
+++ b/files/galaxy/tpv/tools.yml
@@ -331,6 +331,8 @@ tools:
       _GALAXY_JOB_TMP_DIR: '/tmp'
   toolshed.g2.bx.psu.edu/repos/goeckslab/gate_finder/gate_finder/.*:
     inherits: basic_docker_tool
+  toolshed.g2.bx.psu.edu/repos/galaxyp/qupath_roi_splitter/qupath_roi_splitter/.*:
+    inherits: basic_docker_tool
   toolshed.g2.bx.psu.edu/repos/goeckslab/vitessce_spatial/vitessce_spatial/.*:
     inherits: basic_docker_tool
   toolshed.g2.bx.psu.edu/repos/bgruening/cellpose/cellpose/.*: