layout title date default Prototols 2020-12-01 Record keeping A simple electronic lab notebook Data management Molecular cloning Gibson assembly repp-assisted plasmid design Golden gate assembly Freezing and streaking bacteria Miniprep (regular) Gel purification PCR amplification Commonly used E. coli genotypes Cell and organ culture Useful cell numbers in various culture formats Isolation and culture of mouse embryonic salivary glands Isolation and culture of salivary gland epithelial buds Lentivirus packaging and transduction Lentivirus packaging Lentivirus transduction of salivary gland epithelial buds Genome engineering Guide RNA design for gene knockout Immunostaining Whole-mount immunostaining for mouse embryonic organs or 3D spheroid cultures Single-molecule RNA fluorescence in situ hybridization (smFISH) smFISH for tissue culture cells smFISH for whole-mount mouse embryonic organs Protein biochemistry Microtubule co-sedimentation from worm exacts