-
Notifications
You must be signed in to change notification settings - Fork 0
/
Copy pathRUN.sh
20 lines (16 loc) · 1.14 KB
/
RUN.sh
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
#!/bin/bash
# Convert library table to fasta
./convert_lib_to_fasta.sh /Users/plevy/Library/CloudStorage/OneDrive-VHIO/labagros/pierre/proj/upscale/libraries/360RIO136/360RIO136_Lib_v1.0.tsv \
/Users/plevy/Library/CloudStorage/OneDrive-VHIO/labagros/pierre/proj/upscale/libraries/360RIO136/360RIO136_Lib_v1.0.fasta
# Create Bowtie2 indices
# Optional, only if not done before for this library
python3 bowtie2index.py \
/Users/plevy/Library/CloudStorage/OneDrive-VHIO/labagros/pierre/proj/upscale/libraries/360RIO136/360RIO136_Lib_v1.0.fasta
# Run pipeline
python3 main.py \
/Volumes/immuno_share/raw_data/itag/VHIO/360RIO136/Ag_lib_screen/Plasmid_library/AgLib_Lig_1.fastq.gz \
/Volumes/immuno_share/raw_data/itag/VHIO/360RIO136/Ag_lib_screen/Plasmid_library/AgLib_Lig_2.fastq.gz \
/Users/plevy/Library/CloudStorage/OneDrive-VHIO/labagros/pierre/proj/upscale/libraries/360RIO136/360RIO136_Lib_v1.0.fasta
# Join counts
Rscript --vanilla join_counts.R /Users/plevy/Library/CloudStorage/OneDrive-VHIO/labagros/pierre/bin/Aglib_screen/out \
/Users/plevy/Library/CloudStorage/OneDrive-VHIO/labagros/pierre/proj/upscale/libraries/360RIO136/360RIO136_Lib_v1.0.tsv