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sp_wfUseCase.owl
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<?xml version="1.0"?>
<!DOCTYPE rdf:RDF [
<!ENTITY pav "http://purl.org/pav/" >
<!ENTITY terms "http://purl.org/dc/terms/" >
<!ENTITY vann "http://purl.org/vocab/vann/" >
<!ENTITY p-plan "http://purl.org/net/p-plan#" >
<!ENTITY SO "http://purl.org/obo/owl/SO#" >
<!ENTITY bfo "http://www.ifomis.org/bfo/1.1#" >
<!ENTITY owl "http://www.w3.org/2002/07/owl#" >
<!ENTITY obo "http://purl.obolibrary.org/obo/" >
<!ENTITY swrl "http://www.w3.org/2003/11/swrl#" >
<!ENTITY swrlb "http://www.w3.org/2003/11/swrlb#" >
<!ENTITY xsd "http://www.w3.org/2001/XMLSchema#" >
<!ENTITY sp "http://purl.org/net/SMARTprotocol#" >
<!ENTITY owl2xml "http://www.w3.org/2006/12/owl2-xml#" >
<!ENTITY snap "http://www.ifomis.org/bfo/1.1/snap#" >
<!ENTITY span "http://www.ifomis.org/bfo/1.1/span#" >
<!ENTITY ro "http://www.obofoundry.org/ro/ro.owl#" >
<!ENTITY rdfs "http://www.w3.org/2000/01/rdf-schema#" >
<!ENTITY iao "http://purl.obolibrary.org/obo/iao.owl#" >
<!ENTITY npo "http://purl.bioontology.org/ontology/npo#" >
<!ENTITY MSH "http://purl.bioontology.org/ontology/MSH/" >
<!ENTITY rdf "http://www.w3.org/1999/02/22-rdf-syntax-ns#" >
<!ENTITY protege "http://protege.stanford.edu/plugins/owl/protege#" >
<!ENTITY xsp "http://www.owl-ontologies.com/2005/08/07/xsp.owl#" >
<!ENTITY Thesaurus "http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#" >
<!ENTITY Ontology1184060740 "http://www.owl-ontologies.com/Ontology1184060740.owl#" >
<!ENTITY MGEDOntology "http://mged.sourceforge.net/ontologies/MGEDOntology.owl#" >
<!ENTITY miProtocol "http://www.semanticweb.org/ontologies/2013/3/miProtocol.owl#" >
<!ENTITY p1 "http://purl.obolibrary.org/obo/iao/dev/ontology-metadata.owl#" >
<!ENTITY p2 "http://www.semanticweb.org/ontologies/2013/3/Ontology1365706525737.owl#" >
]>
<rdf:RDF xmlns="http://purl.org/net/SMARTprotocol#"
xml:base="http://purl.org/net/SMARTprotocol"
xmlns:p-plan="http://purl.org/net/p-plan#"
xmlns:ro="http://www.obofoundry.org/ro/ro.owl#"
xmlns:p2="http://www.semanticweb.org/ontologies/2013/3/Ontology1365706525737.owl#"
xmlns:p1="&obo;iao/dev/ontology-metadata.owl#"
xmlns:iao="&obo;iao.owl#"
xmlns:snap="http://www.ifomis.org/bfo/1.1/snap#"
xmlns:terms="http://purl.org/dc/terms/"
xmlns:vann="http://purl.org/vocab/vann/"
xmlns:npo="http://purl.bioontology.org/ontology/npo#"
xmlns:bfo="http://www.ifomis.org/bfo/1.1#"
xmlns:Thesaurus="http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#"
xmlns:MSH="http://purl.bioontology.org/ontology/MSH/"
xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#"
xmlns:swrl="http://www.w3.org/2003/11/swrl#"
xmlns:owl2xml="http://www.w3.org/2006/12/owl2-xml#"
xmlns:miProtocol="http://www.semanticweb.org/ontologies/2013/3/miProtocol.owl#"
xmlns:pav="http://purl.org/pav/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:protege="http://protege.stanford.edu/plugins/owl/protege#"
xmlns:sp="http://purl.org/net/SMARTprotocol#"
xmlns:xsp="http://www.owl-ontologies.com/2005/08/07/xsp.owl#"
xmlns:Ontology1184060740="http://www.owl-ontologies.com/Ontology1184060740.owl#"
xmlns:SO="http://purl.org/obo/owl/SO#"
xmlns:obo="http://purl.obolibrary.org/obo/"
xmlns:owl="http://www.w3.org/2002/07/owl#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#"
xmlns:swrlb="http://www.w3.org/2003/11/swrlb#"
xmlns:MGEDOntology="http://mged.sourceforge.net/ontologies/MGEDOntology.owl#"
xmlns:span="http://www.ifomis.org/bfo/1.1/span#">
<owl:Ontology rdf:about="http://purl.org/net/SMARTprotocol">
<rdfs:label xml:lang="en">SP-Workflow Module: Use case</rdfs:label>
<terms:created rdf:datatype="&xsd;date">2013-07-01</terms:created>
<terms:contributor rdf:datatype="&xsd;anyURI">http://delicias.dia.fi.upm.es/members/DGarijo/#me</terms:contributor>
<terms:creator rdf:datatype="&xsd;anyURI">http://oxgiraldo.wordpress.com/</terms:creator>
<terms:contributor rdf:datatype="&xsd;anyURI">http://www.alexandergarcia.name/</terms:contributor>
<terms:contributor rdf:datatype="&xsd;anyURI">http://www.dia.fi.upm.es/index.php?page=oscar-corcho/</terms:contributor>
<terms:license>http://creativecommons.org/licenses/by-nc-sa/2.0/</terms:license>
<vann:preferredNamespaceUri>http://purl.org/net/SMARTprotocol#</vann:preferredNamespaceUri>
<owl:versionInfo xml:lang="en">3.0</owl:versionInfo>
<terms:description xml:lang="en">Here is presented an example showing information that is being modeled in SP-Workflow. The individuals and their corresponding descriptions were extracted from a protocol about extraction of total RNA (the protocols is available here: http://www.biotechniques.com/protocols/Peer-Reviewed_Protocols/Extraction-of-total-RNA-from-freshfrozen-tissue-FT/biotechniques-181792.html)</terms:description>
<terms:title xml:lang="en">SP-Workflow Module: Use case</terms:title>
<vann:preferredNamespacePrefix>sp</vann:preferredNamespacePrefix>
</owl:Ontology>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// Annotation properties
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<!-- http://purl.obolibrary.org/obo/IAO_0000112 -->
<owl:AnnotationProperty rdf:about="&obo;IAO_0000112">
<rdfs:label rdf:datatype="&xsd;string">example of usage</rdfs:label>
<obo:IAO_0000115 rdf:datatype="&xsd;string">A phrase describing how a class name should be used. May also include other kinds of examples that facilitate immediate understanding of a class semantics, such as widely known prototypical subclasses or instances of the class. Although essential for high level terms, examples for low level terms (e.g., Affymetrix HU133 array) are not</obo:IAO_0000115>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000115 -->
<owl:AnnotationProperty rdf:about="&obo;IAO_0000115">
<rdfs:label rdf:datatype="&xsd;string">definition</rdfs:label>
<obo:IAO_0000115 rdf:datatype="&xsd;string">The official definition, explaining the meaning of a class or property. Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions.</obo:IAO_0000115>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000118 -->
<owl:AnnotationProperty rdf:about="&obo;IAO_0000118">
<rdfs:label rdf:datatype="&xsd;string">alternative term</rdfs:label>
<obo:IAO_0000115 rdf:datatype="&xsd;string">An alternative name for a class or property which means the same thing as the preferred name (semantically equivalent)</obo:IAO_0000115>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000119 -->
<owl:AnnotationProperty rdf:about="&obo;IAO_0000119">
<rdfs:label rdf:datatype="&xsd;string">definition source</rdfs:label>
<obo:IAO_0000115 rdf:datatype="&xsd;string">formal citation, e.g. identifier in external database to indicate / attribute source(s) for the definition. Free text indicate / attribute source(s) for the definition. EXAMPLE: Author Name, URI, MeSH Term C04, PUBMED ID, Wiki uri on 31.01.2007</obo:IAO_0000115>
</owl:AnnotationProperty>
<!-- http://purl.obolibrary.org/obo/IAO_0000412 -->
<owl:AnnotationProperty rdf:about="&obo;IAO_0000412">
<rdfs:label rdf:datatype="&xsd;string">imported from</rdfs:label>
<obo:IAO_0000115 rdf:datatype="&xsd;string">For external terms/classes, the ontology from which the term was imported</obo:IAO_0000115>
</owl:AnnotationProperty>
<!-- http://purl.org/dc/terms/description -->
<owl:AnnotationProperty rdf:about="&terms;description"/>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// Object Properties
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<!-- http://purl.obolibrary.org/obo/BFO_0000061 -->
<owl:ObjectProperty rdf:about="&obo;BFO_0000061">
<rdf:type rdf:resource="&owl;TransitiveProperty"/>
<rdfs:label xml:lang="en">precedes</rdfs:label>
<obo:IAO_0000412 xml:lang="en">BFO</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">x precedes y if and only if the time point at which x ends is before or equivalent to the time point at which y starts. Formally: x precedes y iff ω(x) <= α(y), where α is a function that maps a process to a start point, and ω is a function that maps a process to an end point.</obo:IAO_0000115>
</owl:ObjectProperty>
<!-- http://purl.obolibrary.org/obo/BFO_0000062 -->
<owl:ObjectProperty rdf:about="&obo;BFO_0000062">
<rdf:type rdf:resource="&owl;TransitiveProperty"/>
<rdfs:label xml:lang="en">is preceded by</rdfs:label>
<obo:IAO_0000118 rdf:datatype="&xsd;string">preceded by</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">BFO</obo:IAO_0000412>
<owl:inverseOf rdf:resource="&obo;BFO_0000061"/>
</owl:ObjectProperty>
<!-- http://purl.obolibrary.org/obo/RO_0000056 -->
<owl:ObjectProperty rdf:about="&obo;RO_0000056">
<rdfs:label xml:lang="en">participates in</rdfs:label>
<obo:IAO_0000115 xml:lang="en">participates in is a relation that binds a material entity and a protocol instruction.</obo:IAO_0000115>
</owl:ObjectProperty>
<!-- http://purl.obolibrary.org/obo/RO_0001000 -->
<owl:ObjectProperty rdf:about="&obo;RO_0001000">
<rdfs:label>derives from</rdfs:label>
<obo:IAO_0000112>this cell derives from this parent cell (cell division); this nucleus derives from this parent nucleus (nuclear division)</obo:IAO_0000112>
<obo:IAO_0000115>a relation between two distinct material entities, the new entity and the old entity, in which the new entity begins to exist when the old entity ceases to exist, and the new entity inherits the significant portion of the matter of the old entity</obo:IAO_0000115>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasProtocol -->
<owl:ObjectProperty rdf:about="&sp;hasProtocol">
<rdfs:label xml:lang="en">has protocol</rdfs:label>
<obo:IAO_0000115 xml:lang="en">hasProtocol is a relation that binds a laboratory procedure to a experimental protocol.</obo:IAO_0000115>
<rdfs:domain rdf:resource="&Thesaurus;C25294"/>
<rdfs:range rdf:resource="&sp;ExperimentalProtocol"/>
</owl:ObjectProperty>
<!-- http://purl.org/net/SMARTprotocol#hasStep -->
<owl:ObjectProperty rdf:about="&sp;hasStep">
<rdfs:label>has step</rdfs:label>
<obo:IAO_0000115 xml:lang="en">hasStep is a relation that binds a experimental protocol to a protocol instruction.</obo:IAO_0000115>
<rdfs:domain rdf:resource="&sp;ExperimentalProtocol"/>
<rdfs:range rdf:resource="&sp;ProtocolInstruction"/>
</owl:ObjectProperty>
<!-- http://www.obofoundry.org/ro/ro.owl#has_part -->
<owl:ObjectProperty rdf:about="&ro;has_part">
<rdf:type rdf:resource="&owl;TransitiveProperty"/>
<rdfs:label xml:lang="en">has part</rdfs:label>
<obo:IAO_0000412 rdf:datatype="&xsd;string">OBO Relation Ontology</obo:IAO_0000412>
<owl:inverseOf rdf:resource="&ro;part_of"/>
</owl:ObjectProperty>
<!-- http://www.obofoundry.org/ro/ro.owl#part_of -->
<owl:ObjectProperty rdf:about="&ro;part_of">
<rdf:type rdf:resource="&owl;TransitiveProperty"/>
<rdfs:label xml:lang="en">part of</rdfs:label>
<obo:IAO_0000412 rdf:datatype="&xsd;string">OBO Relation Ontology</obo:IAO_0000412>
<obo:IAO_0000115 rdf:datatype="&xsd;string">Parthood as a relation between instances: The primitive instance-level relation p part_of p1 is illustrated in assertions such as: this instance of rhodopsin mediated phototransduction part_of this instance of visual perception. This relation satisfies at least the following standard axioms of mereology: reflexivity (for all p, p part_of p); anti-symmetry (for all p, p1, if p part_of p1 and p1 part_of p then p and p1 are identical); and transitivity (for all p, p1, p2, if p part_of p1 and p1 part_of p2, then p part_of p2). Analogous axioms hold also for parthood as a relation between spatial regions. For parthood as a relation between continuants, these axioms need to be modified to take account of the incorporation of a temporal argument. Thus for example the axiom of transitivity for continuants will assert that if c part_of c1 at t and c1 part_of c2 at t, then also c part_of c2 at t. Parthood as a relation between classes: To define part_of as a relation between classes we again need to distinguish the two cases of continuants and processes, even though the explicit reference to instants of time now falls away. For continuants, we have C part_of C1 if and only if any instance of C at any time is an instance-level part of some instance of C1 at that time, as for example in: cell nucleus part_ of cell.</obo:IAO_0000115>
</owl:ObjectProperty>
<!-- http://www.owl-ontologies.com/Ontology1184060740.owl#has_Part_of -->
<owl:ObjectProperty rdf:about="&Ontology1184060740;has_Part_of"/>
<!--
///////////////////////////////////////////////////////////////////////////////////////
//
// Classes
//
///////////////////////////////////////////////////////////////////////////////////////
-->
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C12801 -->
<owl:Class rdf:about="&Thesaurus;C12801">
<rdfs:label xml:lang="en">tissue</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;OBI_0000671"/>
<obo:IAO_0000119 rdf:datatype="&xsd;string">CDISC</obo:IAO_0000119>
<obo:IAO_0000412 rdf:datatype="&xsd;string">NCI thesaurus</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">An anatomical structure consisting of similarly specialized cells and intercellular matrix, aggregated according to genetically determined spatial relationships, performing a specific function.</obo:IAO_0000115>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C25294 -->
<owl:Class rdf:about="&Thesaurus;C25294">
<rdfs:label xml:lang="en">laboratory procedure</rdfs:label>
<rdfs:subClassOf rdf:resource="http://semanticscience.org/resource/SIO_000999"/>
<obo:IAO_0000412 rdf:datatype="&xsd;string">NCI thesaurus</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">Any procedure that involves testing or manipulating a sample of blood, urine, or other body substance in a laboratory setting.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Test
LBTEST
Laboratory Test
Tests
Lab Test
Lab Tests</obo:IAO_0000118>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C42688 -->
<owl:Class rdf:about="&Thesaurus;C42688">
<rdfs:label xml:lang="en">Instruction</rdfs:label>
<rdfs:label xml:lang="en">instruction</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;IAO_0000300"/>
<obo:IAO_0000115 xml:lang="en">A message describing how something is to be done.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">NCIT</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">step</obo:IAO_0000118>
</owl:Class>
<!-- http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C70830 -->
<owl:Class rdf:about="&Thesaurus;C70830">
<rdfs:label xml:lang="en">solution</rdfs:label>
<rdfs:subClassOf rdf:resource="&snap;MaterialEntity"/>
<owl:disjointWith rdf:resource="&obo;OBI_0000747"/>
<obo:IAO_0000412 rdf:datatype="&xsd;string">NCI thesaurus</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">A homogeneous mixture of two or more substances; frequently (but not necessarily) a liquid solution.</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/CSP/4000-0119 -->
<owl:Class rdf:about="http://purl.bioontology.org/ontology/CSP/4000-0119">
<rdfs:label xml:lang="en">animal tissue</rdfs:label>
<rdfs:subClassOf rdf:resource="&Thesaurus;C12801"/>
<obo:IAO_0000412 xml:lang="en">CRISP</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">nonhuman tissue</obo:IAO_0000118>
<obo:IAO_0000115 xml:lang="en">tissue and cells derived from animals for use in research.</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.bioontology.org/ontology/MSH/D004864 -->
<owl:Class rdf:about="&MSH;D004864">
<rdfs:label xml:lang="en">equipment and supplies</rdfs:label>
<rdfs:subClassOf rdf:resource="&snap;MaterialEntity"/>
<owl:disjointWith rdf:resource="&obo;CHEBI_33893"/>
<owl:disjointWith rdf:resource="&obo;OBI_0000747"/>
<obo:IAO_0000412 rdf:datatype="&xsd;string">Medical Subject Headings (MeSH)</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">Device
Apparatus and Instruments
Device, Medical
Devices, Medical
Instruments and Apparatus
Inventory
Medical Device
Supplies and Equipment
MED DEVICE
EQUIPMENT SUPPLIES
MED DEVICES
APPARATUS INSTRUM
DEVICES MED
DEVICE MED
Devices
Equipment
Inventories
Medical Devices
Supplies</obo:IAO_0000118>
<obo:IAO_0000115 xml:lang="en">Expendable and nonexpendable equipment, supplies, apparatus, and instruments that are used in diagnostic, surgical, therapeutic, scientific, and experimental procedures.</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_17824 -->
<owl:Class rdf:about="&obo;CHEBI_17824">
<rdfs:label xml:lang="en">propan-2-ol</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;CHEBI_35681"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&obo;RO_0000056"/>
<owl:someValuesFrom rdf:resource="&sp;PrecipitationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">A secondary alcohol that is propane in which one of the hydrogens attached to the central carbon is substituted by a hydroxy group.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">i-propanol
C3H8O
Isopropanol
1-methylethyl alcohol
IPA
sec-propanol
i-Propylalkohol
Isopropyl alcohol
2-Propanol
isopropyl alcohol
CC(C)O
Isopropylalkohol
2-hydroxypropane
ISOPROPYL ALCOHOL
1-methylethanol</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_30879 -->
<owl:Class rdf:about="&obo;CHEBI_30879">
<rdfs:label xml:lang="en">alcohol</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">A compound in which a hydroxy group, -OH, is attached to a saturated carbon atom.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">O[*]
an alcohol
HOR</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_33893 -->
<owl:Class rdf:about="&obo;CHEBI_33893">
<rdfs:label xml:lang="en">reagent</rdfs:label>
<rdfs:subClassOf rdf:resource="&snap;MaterialEntity"/>
<owl:disjointWith rdf:resource="&obo;OBI_0000747"/>
<obo:IAO_0000412 rdf:datatype="&xsd;string">ChEBI</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">A substance used in a chemical reaction to detect, measure, examine, or produce other substances.</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_35255 -->
<owl:Class rdf:about="&obo;CHEBI_35255">
<rdfs:label xml:lang="en">chloroform</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;CHEBI_64708"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&obo;RO_0000056"/>
<owl:someValuesFrom rdf:resource="&sp;DenaturationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">A one-carbon compound that is methane in which three of the hydrogens are replaced by chlorines.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">trichloromethane
CHCl3
Trichlormethan
chloroforme
[H]C(Cl)(Cl)Cl
1,1,1-trichloromethane
chloroformium pro narcosi</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_35681 -->
<owl:Class rdf:about="&obo;CHEBI_35681">
<rdfs:label xml:lang="en">secondary alcohol</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;CHEBI_30879"/>
<obo:IAO_0000115 xml:lang="en">A secondary alcohol is a compound in which a hydroxy group, -OH, is attached to a saturated carbon atom which has two other carbon atoms attached to it.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">secondary alcohols
CH2OR2
a secondary alcohol</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/CHEBI_64708 -->
<owl:Class rdf:about="&obo;CHEBI_64708">
<rdfs:label xml:lang="en">one-carbon compound</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;CHEBI_33893"/>
<obo:IAO_0000115 xml:lang="en">An organic molecular entity containing a single carbon atom (C1).</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">CHEBI</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">one-carbon compounds</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000030 -->
<owl:Class rdf:about="&obo;IAO_0000030">
<rdfs:label xml:lang="en">information content entity</rdfs:label>
<rdfs:subClassOf rdf:resource="&snap;GenericallyDependentContinuant"/>
<obo:IAO_0000112 rdf:datatype="&xsd;string">Examples of information content entites include journal articles, data, graphical layouts, and graphs.</obo:IAO_0000112>
<obo:IAO_0000412 rdf:datatype="&xsd;string">OBI</obo:IAO_0000412>
<obo:IAO_0000119 rdf:datatype="&xsd;string">OBI_0000142</obo:IAO_0000119>
<obo:IAO_0000115 rdf:datatype="&xsd;string">an information content entity is an entity that is generically dependent on some artifact and stands in relation of aboutness to some entity</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000300 -->
<owl:Class rdf:about="&obo;IAO_0000300">
<rdfs:label xml:lang="en">textual entity</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;IAO_0000030"/>
<obo:IAO_0000412 rdf:datatype="&xsd;string">OBI</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">A textual entity is a part of a manifestation (FRBR sense), a generically dependent continuant whose concretizations are patterns of glyphs intended to be interpreted as words, formulas, etc.</obo:IAO_0000115>
<obo:IAO_0000112 xml:lang="en">Words, sentences, paragraphs, and the written (non-figure) parts of publications are all textual entities</obo:IAO_0000112>
<obo:IAO_0000118 xml:lang="en">text</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/IAO_0000310 -->
<owl:Class rdf:about="&obo;IAO_0000310">
<rdfs:label xml:lang="en">document</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;IAO_0000030"/>
<obo:IAO_0000115 xml:lang="en">A collection of information content entities intended to be understood together as a whole.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">IAO</obo:IAO_0000412>
<obo:IAO_0000112 xml:lang="en">an experimental protocol</obo:IAO_0000112>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0000671 -->
<owl:Class rdf:about="&obo;OBI_0000671">
<rdfs:label xml:lang="en">sample from organism</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;OBI_0000747"/>
<obo:IAO_0000412 rdf:datatype="&xsd;string">OBI</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">a material obtained from an organism in order to be a representative of the whole</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">sample
specimen
biospecimen</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/OBI_0000747 -->
<owl:Class rdf:about="&obo;OBI_0000747">
<rdfs:label xml:lang="en">material sample</rdfs:label>
<rdfs:subClassOf rdf:resource="&snap;MaterialEntity"/>
<obo:IAO_0000412 rdf:datatype="&xsd;string">OBI</obo:IAO_0000412>
<obo:IAO_0000115 xml:lang="en">A material entity that has the material sample role</obo:IAO_0000115>
<obo:IAO_0000112 xml:lang="en">blood drawn from patient to measure his systemic glucose level. A population of humans with HIV enrolled in a study taken to represent patients with HIV in general.</obo:IAO_0000112>
<obo:IAO_0000118 xml:lang="en">sample population</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009007 -->
<owl:Class rdf:about="&obo;PO_0009007">
<rdfs:label xml:lang="en">plant tissue</rdfs:label>
<rdfs:subClassOf rdf:resource="&Thesaurus;C12801"/>
<obo:IAO_0000115 xml:lang="en">A plant structure (PO:0009011) that consists predominantly of similarly specialized plant cells (PO:0009002) of one or more types. </obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">portion of plant tissue
portion of tissue</obo:IAO_0000118>
<obo:IAO_0000112 xml:lang="en">roots, leaves, flowers, stems.</obo:IAO_0000112>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009010 -->
<owl:Class rdf:about="&obo;PO_0009010">
<rdfs:label xml:lang="en">seed</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A multi-tissue plant structure (PO:0025496) that develops from a plant ovule (PO:0020003) and has as parts a plant embryo (PO:0009009) enclosed in a seed coat (PO:0009088).</obo:IAO_0000115>
<rdfs:comment xml:lang="en">A seed generally develops from an ovule (PO:0020003) after fertilization, but may develop without fertilization in the case of apogamy (e.g., adventitious embryos or somatic embryos). A seed is a reproductive unit of seed plants (gymnosperms, angiosperms, and fossil pteridosperms).</rdfs:comment>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">pyrene
diaspore
semilla
seed tissue</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009046 -->
<owl:Class rdf:about="&obo;PO_0009046">
<rdfs:label xml:lang="en">flower</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A determinate reproductive shoot system that has as part at least one carpel or at least one stamen and does not contain any other determinate shoot system as a part.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">diclinous flower
pistillate flower
staminate flower
basal flower
double flower
female flower
hermaphrodite flower
male flower
perfect flower
imperfect flower
monoclinous flower
flor</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0009047 -->
<owl:Class rdf:about="&obo;PO_0009047">
<rdfs:label xml:lang="en">stem</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A shoot axis (PO:0025029) that is the primary axis of a plant.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Often bears leaves and buds. It is usually above ground, more or less negatively geotropic, and exogenous in origin.</rdfs:comment>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">stalk
trunk
cane
caudices
primocane
scape
caudex
bole
primary stem
tallo
tronco
culm;
eje primario
primary axis</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0025025 -->
<owl:Class rdf:about="&obo;PO_0025025">
<rdfs:label xml:lang="en">root</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A collective plant organ structure (PO:0025007) that produces root apical meristems (PO:0020147) and the plant structures that arise from them.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">root tissue</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0025034 -->
<owl:Class rdf:about="&obo;PO_0025034">
<rdfs:label xml:lang="en">leaf</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">A phyllome (PO:0006001) that is not associated with a reproductive structure. </obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">hoja
leaf sample
leaves
leaf tissue
leaf material
fresh-leaf tissue
fresh leaf tissue
dried leaf tissue
dried leaf sample
dried leaf
Leaf blade</obo:IAO_0000118>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/PO_0025059 -->
<owl:Class rdf:about="&obo;PO_0025059">
<rdfs:label xml:lang="en">ground tissue</rdfs:label>
<rdfs:label xml:lang="en">portion of ground tissue</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;PO_0009007"/>
<obo:IAO_0000115 xml:lang="en">Any portion of plant tissue (PO:0009007) other than epidermis (PO:0005679) or vascular tissue (PO:0009015).</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">plant ontology (PO)</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">portion of ground tissue</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0000948 -->
<owl:Class rdf:about="&obo;UBERON_0000948">
<rdfs:label xml:lang="en">heart</rdfs:label>
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">A myogenic muscular circulatory organ found in the vertebrate cardiovascular system composed of chambers of cardiac muscle. It is the primary circulatory organ.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">chambered heart
vertebrate heart
Herz
branchial heart
Entire heart</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0000955 -->
<owl:Class rdf:about="&obo;UBERON_0000955">
<rdfs:label xml:lang="en">brain</rdfs:label>
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">The brain is the center of the nervous system in all vertebrate, and most invertebrate, animals. Some primitive animals such as jellyfish and starfish have a decentralized nervous system without a brain, while sponges lack any nervous system at all. In vertebrates, the brain is located in the head, protected by the skull and close to the primary sensory apparatus of vision, hearing, balance, taste, and smell[WP].</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002048 -->
<owl:Class rdf:about="&obo;UBERON_0002048">
<rdfs:label xml:lang="en">lung</rdfs:label>
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">Respiration organ that develops as an oupocketing of the esophagus.</obo:IAO_0000115>
<rdfs:comment xml:lang="en">Snakes and limbless lizards typically possess only the right lung as a major respiratory organ; the left lung is greatly reduced, or even absent. Amphisbaenians, however, have the opposite arrangement, with a major left lung, and a reduced or absent right lung [WP]</rdfs:comment>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">pulmo</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002106 -->
<owl:Class rdf:about="&obo;UBERON_0002106">
<rdfs:label xml:lang="en">spleen</rdfs:label>
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">lien</obo:IAO_0000118>
<obo:IAO_0000115 xml:lang="en">the organ that functions to filter blood and to store red corpuscles and platelets</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002107 -->
<owl:Class rdf:about="&obo;UBERON_0002107">
<rdfs:label xml:lang="en">liver</rdfs:label>
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">An exocrine gland which secretes bile and functions in metabolism of protein and carbohydrate and fat, synthesizes substances involved in the clotting of the blood, synthesizes vitamin A, detoxifies poisonous substances, stores glycogen, and breaks down worn-out erythrocytes[GO].</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">jecur
iecur</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.obolibrary.org/obo/UBERON_0002113 -->
<owl:Class rdf:about="&obo;UBERON_0002113">
<rdfs:label xml:lang="en">kidney</rdfs:label>
<rdfs:subClassOf rdf:resource="http://purl.bioontology.org/ontology/CSP/4000-0119"/>
<obo:IAO_0000115 xml:lang="en">A paired organ of the urinary tract which has the production of urine as its primary function.</obo:IAO_0000115>
<obo:IAO_0000412 xml:lang="en">UBERON</obo:IAO_0000412>
<obo:IAO_0000118 xml:lang="en">reniculate kidney</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#CellDisruption -->
<owl:Class rdf:about="&sp;CellDisruption">
<rdfs:label xml:lang="en">cell disruption</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;ProtocolInstruction"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&ro;part_of"/>
<owl:someValuesFrom rdf:resource="&sp;NucleicAcidExtractionProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&obo;BFO_0000061"/>
<owl:someValuesFrom rdf:resource="&sp;DenaturationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">Breaking the cells open, is the first basic step in a DNA extraction. This is commonly achieved by chemical and physical methods-blending, grinding or sonicating the sample.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Cell lysis</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DenaturationReaction -->
<owl:Class rdf:about="&sp;DenaturationReaction">
<rdfs:label xml:lang="en">denaturation reaction</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;ProtocolInstruction"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&obo;BFO_0000062"/>
<owl:someValuesFrom rdf:resource="&sp;CellDisruption"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&obo;BFO_0000061"/>
<owl:someValuesFrom rdf:resource="&sp;PrecipitationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&ro;part_of"/>
<owl:someValuesFrom rdf:resource="&sp;NucleicAcidExtractionProtocol"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">The biological activity of a biopolymer is closely linked to its native structure which is retained only if critical factors such as temperature, pH, solvent and solute are kept within narrow limits. Any alteration of the native structure causing a loss of biological activity is called denaturation.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://www.sigmaaldrich.com/life-science/metabolomics/bioultra-reagents/denaturation.html#sthash.5rKMTmls.dpuf</obo:IAO_0000119>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#DounceHomogenizer -->
<owl:Class rdf:about="&sp;DounceHomogenizer">
<rdfs:label xml:lang="en">dounce homogenizer</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;Homogenizer"/>
<obo:IAO_0000115 xml:lang="en">Shearing caused by a round glass pestle that is manually driven into a glass tube</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">tissue grinder</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#EthanolSolution -->
<owl:Class rdf:about="&sp;EthanolSolution">
<rdfs:label xml:lang="en">ethanol solution</rdfs:label>
<rdfs:subClassOf rdf:resource="&Thesaurus;C70830"/>
<obo:IAO_0000112 xml:lang="en">70% ethanol</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">A homogeneous mixture of ethanol and water.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">http://cameochemicals.noaa.gov/chemical/19240</obo:IAO_0000119>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#ExperimentalProtocol -->
<owl:Class rdf:about="&sp;ExperimentalProtocol">
<rdfs:label xml:lang="en">experimental protocol</rdfs:label>
<rdfs:subClassOf rdf:resource="&obo;IAO_0000310"/>
<obo:IAO_0000115 xml:lang="en">An experimental protocol is a sequence of tasks and operations executed to perform experimental research. The protocols often include equipment, reagents, critical steps, troubleshooting, tips and all the information that facilitates reusability.</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Homogenize -->
<owl:Class rdf:about="&sp;Homogenize">
<rdfs:label xml:lang="en">homogenize</rdfs:label>
<rdfs:subClassOf rdf:resource="&Ontology1184060740;Class_46"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&obo;RO_0000056"/>
<owl:someValuesFrom rdf:resource="&sp;CellDisruption"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">Homogenize is a experimental action. Homogenize is to make uniform or similar.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">make uniform
make similar
unite
integrate
fuse
merge
blend
meld
coalesce
amalgamate
synthesize
combine
join together</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#Homogenizer -->
<owl:Class rdf:about="&sp;Homogenizer">
<rdfs:label xml:lang="en">homogenizer</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;SampleMill"/>
<obo:IAO_0000115 xml:lang="en">A homogenizer is a equipment of laboratory used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. Many different models have been developed using various physical technologies for disruption.</obo:IAO_0000115>
<obo:IAO_0000119 xml:lang="en">https://en.wikipedia.org/wiki/Homogenizer</obo:IAO_0000119>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedGroundTissue -->
<owl:Class rdf:about="&sp;LyophilizedGroundTissue">
<rdfs:label xml:lang="en">lyophilized ground tissue</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried ground tissue
freeze dried ground tissue
</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedLeaf -->
<owl:Class rdf:about="&sp;LyophilizedLeaf">
<rdfs:label xml:lang="en">lyophilized leaf</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried leaf
freeze dried leaf
lyophilized leaf tissue</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedRoot -->
<owl:Class rdf:about="&sp;LyophilizedRoot">
<rdfs:label xml:lang="en">lyophilized root</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried root
freeze dried root
lyophilized root tissue</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedSeed -->
<owl:Class rdf:about="&sp;LyophilizedSeed">
<rdfs:label xml:lang="en">lyophilized seed</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;LyophilizedTissue"/>
<obo:IAO_0000118 xml:lang="en">freeze-dried seed
freeze dried seed
lyophilized seed tissue</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#LyophilizedTissue -->
<owl:Class rdf:about="&sp;LyophilizedTissue">
<rdfs:label xml:lang="en">lyophilized tissue</rdfs:label>
<rdfs:subClassOf rdf:resource="&Thesaurus;C12801"/>
<obo:IAO_0000112 xml:lang="en">lyophilized leaf, lyophilized root, lyophilized seed</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">lyophilized tissue is a tissue dried by freezing in a high vacuum</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#NucleicAcidExtractionProcedure -->
<owl:Class rdf:about="&sp;NucleicAcidExtractionProcedure">
<rdfs:label xml:lang="en">nucleic acid extraction procedure</rdfs:label>
<rdfs:subClassOf rdf:resource="&Thesaurus;C25294"/>
<obo:IAO_0000112 xml:lang="en">DNA extraction procedure, RNA extraction procedure</obo:IAO_0000112>
<obo:IAO_0000115 xml:lang="en">Nucleic acid extraction procedure is a subclass of laboratory procedure used to recover the nucleic acid fraction of an input material entity.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">Nucleic acid isolation procedure</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#NucleicAcidExtractionProtocol -->
<owl:Class rdf:about="&sp;NucleicAcidExtractionProtocol">
<rdfs:label xml:lang="en">nucleic acid extraction protocol</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;ExperimentalProtocol"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&sp;hasStep"/>
<owl:someValuesFrom rdf:resource="&sp;CellDisruption"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&sp;hasStep"/>
<owl:someValuesFrom rdf:resource="&sp;PrecipitationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&sp;hasStep"/>
<owl:someValuesFrom rdf:resource="&sp;DenaturationReaction"/>
</owl:Restriction>
</rdfs:subClassOf>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&sp;hasStep"/>
<owl:someValuesFrom rdf:resource="&sp;WashingNucleicAcids"/>
</owl:Restriction>
</rdfs:subClassOf>
<obo:IAO_0000115 xml:lang="en">Nucleic acid extraction protocol is a subclass of laboratory protocol. A nucleic acid extraction protocol is a step by step description of a procedure used to recover the nucleic acid fraction of an input material entity.</obo:IAO_0000115>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PhysicalDisruptionEquipmentAndSupplies -->
<owl:Class rdf:about="&sp;PhysicalDisruptionEquipmentAndSupplies">
<rdfs:label xml:lang="en">physical disruption equipment and supplies</rdfs:label>
<rdfs:subClassOf rdf:resource="&MSH;D004864"/>
<rdfs:subClassOf>
<owl:Restriction>
<owl:onProperty rdf:resource="&obo;RO_0000056"/>
<owl:someValuesFrom rdf:resource="&sp;CellDisruption"/>
</owl:Restriction>
</rdfs:subClassOf>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PolytronHomogenizer -->
<owl:Class rdf:about="&sp;PolytronHomogenizer">
<rdfs:label xml:lang="en">polytron homogenizer</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;Homogenizer"/>
<obo:IAO_0000115 xml:lang="en">Polytron homogenizer is a instrument used for pulverizing plant and animal tissue, grain and seed, soil extraction, DNA/RNA extraction.</obo:IAO_0000115>
<obo:IAO_0000118 xml:lang="en">polytron
poly-tron mixer
polytron mixer</obo:IAO_0000118>
</owl:Class>
<!-- http://purl.org/net/SMARTprotocol#PotterElvehjemHomogenizer -->
<owl:Class rdf:about="&sp;PotterElvehjemHomogenizer">
<rdfs:label xml:lang="en">potter-elvehjem homogenizer</rdfs:label>
<rdfs:subClassOf rdf:resource="&sp;Homogenizer"/>
<obo:IAO_0000115 xml:lang="en">Shearing is caused by a PTFE- coated pestle that is manually or mechanically driven into a conical vessel</obo:IAO_0000115>
</owl:Class>