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Jeff did some digging around and found some weird behavior between bbmerge and bowtie2 where paired reads that we'd expect to be merged aren't always merged and vice versa. It would be good to know how often this happens, and see if we can prevent this behavior from happening by changing parameters in one or both of the softwares.
The text was updated successfully, but these errors were encountered:
willbradshaw
changed the title
Investigate weird behavior between bbmerge and bowtie2
Investigate and improve the pipeline's read-merging behavior
Dec 17, 2024
Further investigation suggests that we do have a genuine issue here, where about 5-10% of reads are being incorrectly merged by BBMerge in a way that other approaches to merging can handle better. While this isn't a terrible failure rate, we should still investigate and see if we can improve the situation.
Jeff did some digging around and found some weird behavior between bbmerge and bowtie2 where paired reads that we'd expect to be merged aren't always merged and vice versa. It would be good to know how often this happens, and see if we can prevent this behavior from happening by changing parameters in one or both of the softwares.
The text was updated successfully, but these errors were encountered: