From a0719cd94f9e2fb4cb16ce2f342083bf23b3cac9 Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Thu, 24 Aug 2023 10:59:48 +0200 Subject: [PATCH 1/8] fixing issue where Darwin tool cannot handle decimals --- protocols/CoverageCalculations_gvcf.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/protocols/CoverageCalculations_gvcf.sh b/protocols/CoverageCalculations_gvcf.sh index e56c8bc5..bee21e2c 100755 --- a/protocols/CoverageCalculations_gvcf.sh +++ b/protocols/CoverageCalculations_gvcf.sh @@ -125,7 +125,7 @@ then grep -v "NC_001422.1" "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt" > "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt.tmp" echo "phiX is removed for ${externalSampleID}.${perBase} perBase" - mv -v "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt.tmp" "${projectResultsDir}/coverage/CoveragePerBase/${Gender,,}/${externalSampleID}.${perBase}.coveragePerBase.txt" + awk '{if (NR>1){printf "%s\t%s\t%s\t%s\t%.0f\t%s\t%s\t%s\t%s\n",$1,$2,$3,$4,$5,$6,$7,$8,$9}else {print $0}}' "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt.tmp" > "${projectResultsDir}/coverage/CoveragePerBase/${Gender,,}/${externalSampleID}.${perBase}.coveragePerBase.txt" echo "coveragePerTarget file can be found here: ${projectResultsDir}/coverage/CoveragePerBase/${Gender,,}/${externalSampleID}.${perBase}.coveragePerBase.txt" From e302f38a49fa207acb67b2f2f4c5711960cea732 Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Mon, 28 Aug 2023 08:49:54 +0200 Subject: [PATCH 2/8] coveragePerBase decimals has been changed back to original state, so it needs to change in the truth file as well --- ...Test_v1.coveragePerBase.selection_True.txt | 198 +++++++++--------- 1 file changed, 99 insertions(+), 99 deletions(-) diff --git a/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt b/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt index f2eb6aeb..320d11ce 100644 --- a/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt +++ b/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt @@ -1,100 +1,100 @@ Index Chr Chr Position Start Description Min Counts CDS Contig -1 2 197550 AC079779.7 33.0 CDS 1 -2 2 197551 AC079779.7 33.0 CDS 1 -3 2 197552 AC079779.7 33.0 CDS 1 -4 2 197553 AC079779.7 33.0 CDS 1 -5 2 197554 AC079779.7 33.0 CDS 1 -6 2 197555 AC079779.7 33.0 CDS 1 -7 2 197556 AC079779.7 33.0 CDS 1 -8 2 197557 AC079779.7 33.0 CDS 1 -9 2 197558 AC079779.7 33.0 CDS 1 -10 2 197559 AC079779.7 33.0 CDS 1 -11 2 197560 AC079779.7 33.0 CDS 1 -12 2 197561 AC079779.7 33.0 CDS 1 -13 2 197562 AC079779.7 33.0 CDS 1 -14 2 197563 AC079779.7 33.0 CDS 1 -15 2 197564 AC079779.7 36.0 CDS 1 -16 2 197565 AC079779.7 36.0 CDS 1 -17 2 197566 AC079779.7 36.0 CDS 1 -18 2 197567 AC079779.7 36.0 CDS 1 -19 2 197568 AC079779.7 31.0 CDS 1 -20 2 197569 AC079779.7 31.0 CDS 1 -21 2 197570 AC079779.7 31.0 CDS 1 -22 2 197571 AC079779.7 31.0 CDS 1 -23 2 197572 AC079779.7 31.0 CDS 1 -24 2 197573 AC079779.7 31.0 CDS 1 -25 2 197574 AC079779.7 31.0 CDS 1 -26 2 197575 AC079779.7 31.0 CDS 1 -27 2 197576 AC079779.7 31.0 CDS 1 -28 2 197577 AC079779.7 31.0 CDS 1 -29 2 197578 AC079779.7 31.0 CDS 1 -30 2 197579 AC079779.7 31.0 CDS 1 -31 2 197580 AC079779.7 31.0 CDS 1 -32 2 197581 AC079779.7 31.0 CDS 1 -33 2 197582 AC079779.7 31.0 CDS 1 -34 2 197583 AC079779.7 31.0 CDS 1 -35 2 197584 AC079779.7 28.0 CDS 1 -36 2 197585 AC079779.7 28.0 CDS 1 -37 2 197586 AC079779.7 28.0 CDS 1 -38 2 197587 AC079779.7 28.0 CDS 1 -39 2 197588 AC079779.7 28.0 CDS 1 -40 2 197589 AC079779.7 28.0 CDS 1 -41 2 197590 AC079779.7 28.0 CDS 1 -42 2 197591 AC079779.7 28.0 CDS 1 -43 2 197592 AC079779.7 28.0 CDS 1 -44 2 197593 AC079779.7 27.0 CDS 1 -45 2 197594 AC079779.7 26.0 CDS 1 -46 2 197595 AC079779.7 26.0 CDS 1 -47 2 197596 AC079779.7 26.0 CDS 1 -48 2 197597 AC079779.7 25.0 CDS 1 -49 2 197598 AC079779.7 25.0 CDS 1 -52329 Y 2803782 ZFY 0.0 CDS 1 -52330 Y 2803783 ZFY 0.0 CDS 1 -52331 Y 2803784 ZFY 0.0 CDS 1 -52332 Y 2803785 ZFY 0.0 CDS 1 -52333 Y 2803786 ZFY 0.0 CDS 1 -52334 Y 2803787 ZFY 0.0 CDS 1 -52335 Y 2803788 ZFY 0.0 CDS 1 -52336 Y 2803789 ZFY 0.0 CDS 1 -52337 Y 2803790 ZFY 0.0 CDS 1 -52338 Y 2803791 ZFY 0.0 CDS 1 -52339 Y 2803792 ZFY 0.0 CDS 1 -52340 Y 2803793 ZFY 0.0 CDS 1 -52341 Y 2803794 ZFY 0.0 CDS 1 -52342 Y 2803795 ZFY 0.0 CDS 1 -52343 Y 2803796 ZFY 0.0 CDS 1 -52344 Y 2803797 ZFY 0.0 CDS 1 -52345 Y 2803798 ZFY 0.0 CDS 1 -52346 Y 2803799 ZFY 0.0 CDS 1 -52347 Y 2803800 ZFY 0.0 CDS 1 -52348 Y 2803801 ZFY 0.0 CDS 1 -52349 Y 2803802 ZFY 0.0 CDS 1 -52350 Y 2803803 ZFY 0.0 CDS 1 -52351 Y 2803804 ZFY 0.0 CDS 1 -52352 Y 2803805 ZFY 0.0 CDS 1 -52353 Y 2803806 ZFY 0.0 CDS 1 -52354 Y 2803807 ZFY 0.0 CDS 1 -52355 Y 2803808 ZFY 0.0 CDS 1 -52356 Y 2803809 ZFY 0.0 CDS 1 -52357 Y 2803810 ZFY 0.0 CDS 1 -52358 Y 2803811 ZFY 0.0 CDS 1 -52359 Y 2803812 ZFY 0.0 CDS 1 -52360 Y 2803813 ZFY 0.0 CDS 1 -52361 Y 2803814 ZFY 0.0 CDS 1 -52362 Y 2803815 ZFY 0.0 CDS 1 -52363 Y 2803816 ZFY 0.0 CDS 1 -52364 Y 2803817 ZFY 0.0 CDS 1 -52365 Y 2803818 ZFY 0.0 CDS 1 -52366 Y 2803819 ZFY 0.0 CDS 1 -52367 Y 2803820 ZFY 0.0 CDS 1 -52368 Y 2803821 ZFY 0.0 CDS 1 -52369 Y 2803822 ZFY 0.0 CDS 1 -52370 Y 2803823 ZFY 0.0 CDS 1 -52371 Y 2803824 ZFY 0.0 CDS 1 -52372 Y 2803825 ZFY 0.0 CDS 1 -52373 Y 2803826 ZFY 0.0 CDS 1 -52374 Y 2803827 ZFY 0.0 CDS 1 -52375 Y 2803828 ZFY 0.0 CDS 1 -52376 Y 2803829 ZFY 0.0 CDS 1 -52377 Y 2803830 ZFY 0.0 CDS 1 -52378 Y 2803831 ZFY 0.0 CDS 1 +1 2 197550 AC079779.7 33 CDS 1 +2 2 197551 AC079779.7 33 CDS 1 +3 2 197552 AC079779.7 33 CDS 1 +4 2 197553 AC079779.7 33 CDS 1 +5 2 197554 AC079779.7 33 CDS 1 +6 2 197555 AC079779.7 33 CDS 1 +7 2 197556 AC079779.7 33 CDS 1 +8 2 197557 AC079779.7 33 CDS 1 +9 2 197558 AC079779.7 33 CDS 1 +10 2 197559 AC079779.7 33 CDS 1 +11 2 197560 AC079779.7 33 CDS 1 +12 2 197561 AC079779.7 33 CDS 1 +13 2 197562 AC079779.7 33 CDS 1 +14 2 197563 AC079779.7 33 CDS 1 +15 2 197564 AC079779.7 36 CDS 1 +16 2 197565 AC079779.7 36 CDS 1 +17 2 197566 AC079779.7 36 CDS 1 +18 2 197567 AC079779.7 36 CDS 1 +19 2 197568 AC079779.7 31 CDS 1 +20 2 197569 AC079779.7 31 CDS 1 +21 2 197570 AC079779.7 31 CDS 1 +22 2 197571 AC079779.7 31 CDS 1 +23 2 197572 AC079779.7 31 CDS 1 +24 2 197573 AC079779.7 31 CDS 1 +25 2 197574 AC079779.7 31 CDS 1 +26 2 197575 AC079779.7 31 CDS 1 +27 2 197576 AC079779.7 31 CDS 1 +28 2 197577 AC079779.7 31 CDS 1 +29 2 197578 AC079779.7 31 CDS 1 +30 2 197579 AC079779.7 31 CDS 1 +31 2 197580 AC079779.7 31 CDS 1 +32 2 197581 AC079779.7 31 CDS 1 +33 2 197582 AC079779.7 31 CDS 1 +34 2 197583 AC079779.7 31 CDS 1 +35 2 197584 AC079779.7 28 CDS 1 +36 2 197585 AC079779.7 28 CDS 1 +37 2 197586 AC079779.7 28 CDS 1 +38 2 197587 AC079779.7 28 CDS 1 +39 2 197588 AC079779.7 28 CDS 1 +40 2 197589 AC079779.7 28 CDS 1 +41 2 197590 AC079779.7 28 CDS 1 +42 2 197591 AC079779.7 28 CDS 1 +43 2 197592 AC079779.7 28 CDS 1 +44 2 197593 AC079779.7 27 CDS 1 +45 2 197594 AC079779.7 26 CDS 1 +46 2 197595 AC079779.7 26 CDS 1 +47 2 197596 AC079779.7 26 CDS 1 +48 2 197597 AC079779.7 25 CDS 1 +49 2 197598 AC079779.7 25 CDS 1 +52329 Y 2803782 ZFY 0 CDS 1 +52330 Y 2803783 ZFY 0 CDS 1 +52331 Y 2803784 ZFY 0 CDS 1 +52332 Y 2803785 ZFY 0 CDS 1 +52333 Y 2803786 ZFY 0 CDS 1 +52334 Y 2803787 ZFY 0 CDS 1 +52335 Y 2803788 ZFY 0 CDS 1 +52336 Y 2803789 ZFY 0 CDS 1 +52337 Y 2803790 ZFY 0 CDS 1 +52338 Y 2803791 ZFY 0 CDS 1 +52339 Y 2803792 ZFY 0 CDS 1 +52340 Y 2803793 ZFY 0 CDS 1 +52341 Y 2803794 ZFY 0 CDS 1 +52342 Y 2803795 ZFY 0 CDS 1 +52343 Y 2803796 ZFY 0 CDS 1 +52344 Y 2803797 ZFY 0 CDS 1 +52345 Y 2803798 ZFY 0 CDS 1 +52346 Y 2803799 ZFY 0 CDS 1 +52347 Y 2803800 ZFY 0 CDS 1 +52348 Y 2803801 ZFY 0 CDS 1 +52349 Y 2803802 ZFY 0 CDS 1 +52350 Y 2803803 ZFY 0 CDS 1 +52351 Y 2803804 ZFY 0 CDS 1 +52352 Y 2803805 ZFY 0 CDS 1 +52353 Y 2803806 ZFY 0 CDS 1 +52354 Y 2803807 ZFY 0 CDS 1 +52355 Y 2803808 ZFY 0 CDS 1 +52356 Y 2803809 ZFY 0 CDS 1 +52357 Y 2803810 ZFY 0 CDS 1 +52358 Y 2803811 ZFY 0 CDS 1 +52359 Y 2803812 ZFY 0 CDS 1 +52360 Y 2803813 ZFY 0 CDS 1 +52361 Y 2803814 ZFY 0 CDS 1 +52362 Y 2803815 ZFY 0 CDS 1 +52363 Y 2803816 ZFY 0 CDS 1 +52364 Y 2803817 ZFY 0 CDS 1 +52365 Y 2803818 ZFY 0 CDS 1 +52366 Y 2803819 ZFY 0 CDS 1 +52367 Y 2803820 ZFY 0 CDS 1 +52368 Y 2803821 ZFY 0 CDS 1 +52369 Y 2803822 ZFY 0 CDS 1 +52370 Y 2803823 ZFY 0 CDS 1 +52371 Y 2803824 ZFY 0 CDS 1 +52372 Y 2803825 ZFY 0 CDS 1 +52373 Y 2803826 ZFY 0 CDS 1 +52374 Y 2803827 ZFY 0 CDS 1 +52375 Y 2803828 ZFY 0 CDS 1 +52376 Y 2803829 ZFY 0 CDS 1 +52377 Y 2803830 ZFY 0 CDS 1 +52378 Y 2803831 ZFY 0 CDS 1 From b16cc7c93ad8d8bc82050211effa83423463713d Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Wed, 30 Aug 2023 12:42:06 +0200 Subject: [PATCH 3/8] force build --- README.md | 1 - 1 file changed, 1 deletion(-) diff --git a/README.md b/README.md index 89e7a6fe..ff0f2af3 100755 --- a/README.md +++ b/README.md @@ -20,4 +20,3 @@ Based on quality thresholds from the GATK "best practices"5, the SNPs 3. Tarasov A et al. (2015). Sambamba: Fast processing of NGS alignment formats.
4. McKenna A et al. (2010). The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data.
5. Van der Auwera GA et al. (2013). From FastQ data to high confidence variant calls: the Genome Analysis Toolkit best practices pipeline.
- From 8dc0eae9a088bcfddc5c2b3ecf7dd6068ede456e Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Wed, 30 Aug 2023 13:19:16 +0200 Subject: [PATCH 4/8] trigger builkd --- README.md | 1 + 1 file changed, 1 insertion(+) diff --git a/README.md b/README.md index ff0f2af3..89e7a6fe 100755 --- a/README.md +++ b/README.md @@ -20,3 +20,4 @@ Based on quality thresholds from the GATK "best practices"5, the SNPs 3. Tarasov A et al. (2015). Sambamba: Fast processing of NGS alignment formats.
4. McKenna A et al. (2010). The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data.
5. Van der Auwera GA et al. (2013). From FastQ data to high confidence variant calls: the Genome Analysis Toolkit best practices pipeline.
+ From 3b9ce8c123abb9abe186055fde028848f72e63da Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Wed, 30 Aug 2023 16:57:13 +0200 Subject: [PATCH 5/8] repairing this HUUUUGE bug in git where changing the capitalization of your filenames should never occur once it is in the repo already --- workflow_DRAGEN.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflow_DRAGEN.csv b/workflow_DRAGEN.csv index 3d765e6d..eaf7b04d 100644 --- a/workflow_DRAGEN.csv +++ b/workflow_DRAGEN.csv @@ -1,6 +1,6 @@ step,protocol,dependencies s01_PrepareDragenData,protocols/PrepareDragenData.sh, -s01b_processDragenTrendAnalysis,protocols/processDragenTrendAnalysis.sh,s01_PrepareDragenData +s01b_processDragenTrendanalysis,protocols/processDragenTrendanalysis.sh,s01_PrepareDragenData s02_CoverageCalculations,protocols/CoverageCalculations_gvcf.sh,s01_PrepareDragenData;variantCalls=sampleDragenRawVariantCalls;merge=noMerge s03_AnnotateVcf,protocols/AnnotateVcf_perSample.sh,s01_PrepareDragenData;variantCalls=sampleDragenVariantCalls s04_SnpEff,protocols/SnpEff_perSample.sh,s03_AnnotateVcf From dc34459d9968593c8a4834e61d23543c0f35fc92 Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Wed, 30 Aug 2023 17:05:40 +0200 Subject: [PATCH 6/8] fix capitalization issue --- ...ssDragenTrendanalysis.sh => ProcessDragenTrendAnalysis.sh} | 0 workflow_DRAGEN.csv | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename protocols/{processDragenTrendanalysis.sh => ProcessDragenTrendAnalysis.sh} (100%) diff --git a/protocols/processDragenTrendanalysis.sh b/protocols/ProcessDragenTrendAnalysis.sh similarity index 100% rename from protocols/processDragenTrendanalysis.sh rename to protocols/ProcessDragenTrendAnalysis.sh diff --git a/workflow_DRAGEN.csv b/workflow_DRAGEN.csv index eaf7b04d..75d26de0 100644 --- a/workflow_DRAGEN.csv +++ b/workflow_DRAGEN.csv @@ -1,9 +1,9 @@ step,protocol,dependencies s01_PrepareDragenData,protocols/PrepareDragenData.sh, -s01b_processDragenTrendanalysis,protocols/processDragenTrendanalysis.sh,s01_PrepareDragenData +s01b_ProcessDragenTrendAnalysis,protocols/ProcessDragenTrendAnalysis.sh,s01_PrepareDragenData s02_CoverageCalculations,protocols/CoverageCalculations_gvcf.sh,s01_PrepareDragenData;variantCalls=sampleDragenRawVariantCalls;merge=noMerge s03_AnnotateVcf,protocols/AnnotateVcf_perSample.sh,s01_PrepareDragenData;variantCalls=sampleDragenVariantCalls s04_SnpEff,protocols/SnpEff_perSample.sh,s03_AnnotateVcf s08_Gavin,protocols/Gavin.sh,s04_SnpEff;inputFile=sampleVariantCallsSnpEff_Annotated s09_ReheaderVcf,protocols/ReheaderVCF.sh,s08_Gavin -s10_PipelineFinished,protocols/PipelineFinished.sh,s01b_processDragenTrendAnalysis;s02_CoverageCalculations;s09_ReheaderVcf \ No newline at end of file +s10_PipelineFinished,protocols/PipelineFinished.sh,s01b_processDragenTrendAnalysis;s02_CoverageCalculations;s09_ReheaderVcf From 6b88f30500bde38dfba8d1a17c0456b38092e2f8 Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Wed, 30 Aug 2023 17:14:40 +0200 Subject: [PATCH 7/8] removing 2 empty tabs at the end of the coveragePerBase output --- protocols/CoverageCalculations_gvcf.sh | 2 +- ...Test_v1.coveragePerBase.selection_True.txt | 198 +++++++++--------- 2 files changed, 100 insertions(+), 100 deletions(-) diff --git a/protocols/CoverageCalculations_gvcf.sh b/protocols/CoverageCalculations_gvcf.sh index bee21e2c..1ef1cc6a 100755 --- a/protocols/CoverageCalculations_gvcf.sh +++ b/protocols/CoverageCalculations_gvcf.sh @@ -125,7 +125,7 @@ then grep -v "NC_001422.1" "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt" > "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt.tmp" echo "phiX is removed for ${externalSampleID}.${perBase} perBase" - awk '{if (NR>1){printf "%s\t%s\t%s\t%s\t%.0f\t%s\t%s\t%s\t%s\n",$1,$2,$3,$4,$5,$6,$7,$8,$9}else {print $0}}' "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt.tmp" > "${projectResultsDir}/coverage/CoveragePerBase/${Gender,,}/${externalSampleID}.${perBase}.coveragePerBase.txt" + awk '{if (NR>1){printf "%s\t%s\t%s\t%s\t%.0f\t%s\t%s\n",$1,$2,$3,$4,$5,$6,$7}else {print $0}}' "${intermediateDir}/${externalSampleID}.${perBase}.coveragePerBase.txt.tmp" > "${projectResultsDir}/coverage/CoveragePerBase/${Gender,,}/${externalSampleID}.${perBase}.coveragePerBase.txt" echo "coveragePerTarget file can be found here: ${projectResultsDir}/coverage/CoveragePerBase/${Gender,,}/${externalSampleID}.${perBase}.coveragePerBase.txt" diff --git a/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt b/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt index 320d11ce..d615b866 100644 --- a/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt +++ b/test/results/PlatinumSample_NA12878.NGS_DNA_Test_v1.coveragePerBase.selection_True.txt @@ -1,100 +1,100 @@ Index Chr Chr Position Start Description Min Counts CDS Contig -1 2 197550 AC079779.7 33 CDS 1 -2 2 197551 AC079779.7 33 CDS 1 -3 2 197552 AC079779.7 33 CDS 1 -4 2 197553 AC079779.7 33 CDS 1 -5 2 197554 AC079779.7 33 CDS 1 -6 2 197555 AC079779.7 33 CDS 1 -7 2 197556 AC079779.7 33 CDS 1 -8 2 197557 AC079779.7 33 CDS 1 -9 2 197558 AC079779.7 33 CDS 1 -10 2 197559 AC079779.7 33 CDS 1 -11 2 197560 AC079779.7 33 CDS 1 -12 2 197561 AC079779.7 33 CDS 1 -13 2 197562 AC079779.7 33 CDS 1 -14 2 197563 AC079779.7 33 CDS 1 -15 2 197564 AC079779.7 36 CDS 1 -16 2 197565 AC079779.7 36 CDS 1 -17 2 197566 AC079779.7 36 CDS 1 -18 2 197567 AC079779.7 36 CDS 1 -19 2 197568 AC079779.7 31 CDS 1 -20 2 197569 AC079779.7 31 CDS 1 -21 2 197570 AC079779.7 31 CDS 1 -22 2 197571 AC079779.7 31 CDS 1 -23 2 197572 AC079779.7 31 CDS 1 -24 2 197573 AC079779.7 31 CDS 1 -25 2 197574 AC079779.7 31 CDS 1 -26 2 197575 AC079779.7 31 CDS 1 -27 2 197576 AC079779.7 31 CDS 1 -28 2 197577 AC079779.7 31 CDS 1 -29 2 197578 AC079779.7 31 CDS 1 -30 2 197579 AC079779.7 31 CDS 1 -31 2 197580 AC079779.7 31 CDS 1 -32 2 197581 AC079779.7 31 CDS 1 -33 2 197582 AC079779.7 31 CDS 1 -34 2 197583 AC079779.7 31 CDS 1 -35 2 197584 AC079779.7 28 CDS 1 -36 2 197585 AC079779.7 28 CDS 1 -37 2 197586 AC079779.7 28 CDS 1 -38 2 197587 AC079779.7 28 CDS 1 -39 2 197588 AC079779.7 28 CDS 1 -40 2 197589 AC079779.7 28 CDS 1 -41 2 197590 AC079779.7 28 CDS 1 -42 2 197591 AC079779.7 28 CDS 1 -43 2 197592 AC079779.7 28 CDS 1 -44 2 197593 AC079779.7 27 CDS 1 -45 2 197594 AC079779.7 26 CDS 1 -46 2 197595 AC079779.7 26 CDS 1 -47 2 197596 AC079779.7 26 CDS 1 -48 2 197597 AC079779.7 25 CDS 1 -49 2 197598 AC079779.7 25 CDS 1 -52329 Y 2803782 ZFY 0 CDS 1 -52330 Y 2803783 ZFY 0 CDS 1 -52331 Y 2803784 ZFY 0 CDS 1 -52332 Y 2803785 ZFY 0 CDS 1 -52333 Y 2803786 ZFY 0 CDS 1 -52334 Y 2803787 ZFY 0 CDS 1 -52335 Y 2803788 ZFY 0 CDS 1 -52336 Y 2803789 ZFY 0 CDS 1 -52337 Y 2803790 ZFY 0 CDS 1 -52338 Y 2803791 ZFY 0 CDS 1 -52339 Y 2803792 ZFY 0 CDS 1 -52340 Y 2803793 ZFY 0 CDS 1 -52341 Y 2803794 ZFY 0 CDS 1 -52342 Y 2803795 ZFY 0 CDS 1 -52343 Y 2803796 ZFY 0 CDS 1 -52344 Y 2803797 ZFY 0 CDS 1 -52345 Y 2803798 ZFY 0 CDS 1 -52346 Y 2803799 ZFY 0 CDS 1 -52347 Y 2803800 ZFY 0 CDS 1 -52348 Y 2803801 ZFY 0 CDS 1 -52349 Y 2803802 ZFY 0 CDS 1 -52350 Y 2803803 ZFY 0 CDS 1 -52351 Y 2803804 ZFY 0 CDS 1 -52352 Y 2803805 ZFY 0 CDS 1 -52353 Y 2803806 ZFY 0 CDS 1 -52354 Y 2803807 ZFY 0 CDS 1 -52355 Y 2803808 ZFY 0 CDS 1 -52356 Y 2803809 ZFY 0 CDS 1 -52357 Y 2803810 ZFY 0 CDS 1 -52358 Y 2803811 ZFY 0 CDS 1 -52359 Y 2803812 ZFY 0 CDS 1 -52360 Y 2803813 ZFY 0 CDS 1 -52361 Y 2803814 ZFY 0 CDS 1 -52362 Y 2803815 ZFY 0 CDS 1 -52363 Y 2803816 ZFY 0 CDS 1 -52364 Y 2803817 ZFY 0 CDS 1 -52365 Y 2803818 ZFY 0 CDS 1 -52366 Y 2803819 ZFY 0 CDS 1 -52367 Y 2803820 ZFY 0 CDS 1 -52368 Y 2803821 ZFY 0 CDS 1 -52369 Y 2803822 ZFY 0 CDS 1 -52370 Y 2803823 ZFY 0 CDS 1 -52371 Y 2803824 ZFY 0 CDS 1 -52372 Y 2803825 ZFY 0 CDS 1 -52373 Y 2803826 ZFY 0 CDS 1 -52374 Y 2803827 ZFY 0 CDS 1 -52375 Y 2803828 ZFY 0 CDS 1 -52376 Y 2803829 ZFY 0 CDS 1 -52377 Y 2803830 ZFY 0 CDS 1 -52378 Y 2803831 ZFY 0 CDS 1 +1 2 197550 AC079779.7 33 CDS 1 +2 2 197551 AC079779.7 33 CDS 1 +3 2 197552 AC079779.7 33 CDS 1 +4 2 197553 AC079779.7 33 CDS 1 +5 2 197554 AC079779.7 33 CDS 1 +6 2 197555 AC079779.7 33 CDS 1 +7 2 197556 AC079779.7 33 CDS 1 +8 2 197557 AC079779.7 33 CDS 1 +9 2 197558 AC079779.7 33 CDS 1 +10 2 197559 AC079779.7 33 CDS 1 +11 2 197560 AC079779.7 33 CDS 1 +12 2 197561 AC079779.7 33 CDS 1 +13 2 197562 AC079779.7 33 CDS 1 +14 2 197563 AC079779.7 33 CDS 1 +15 2 197564 AC079779.7 36 CDS 1 +16 2 197565 AC079779.7 36 CDS 1 +17 2 197566 AC079779.7 36 CDS 1 +18 2 197567 AC079779.7 36 CDS 1 +19 2 197568 AC079779.7 31 CDS 1 +20 2 197569 AC079779.7 31 CDS 1 +21 2 197570 AC079779.7 31 CDS 1 +22 2 197571 AC079779.7 31 CDS 1 +23 2 197572 AC079779.7 31 CDS 1 +24 2 197573 AC079779.7 31 CDS 1 +25 2 197574 AC079779.7 31 CDS 1 +26 2 197575 AC079779.7 31 CDS 1 +27 2 197576 AC079779.7 31 CDS 1 +28 2 197577 AC079779.7 31 CDS 1 +29 2 197578 AC079779.7 31 CDS 1 +30 2 197579 AC079779.7 31 CDS 1 +31 2 197580 AC079779.7 31 CDS 1 +32 2 197581 AC079779.7 31 CDS 1 +33 2 197582 AC079779.7 31 CDS 1 +34 2 197583 AC079779.7 31 CDS 1 +35 2 197584 AC079779.7 28 CDS 1 +36 2 197585 AC079779.7 28 CDS 1 +37 2 197586 AC079779.7 28 CDS 1 +38 2 197587 AC079779.7 28 CDS 1 +39 2 197588 AC079779.7 28 CDS 1 +40 2 197589 AC079779.7 28 CDS 1 +41 2 197590 AC079779.7 28 CDS 1 +42 2 197591 AC079779.7 28 CDS 1 +43 2 197592 AC079779.7 28 CDS 1 +44 2 197593 AC079779.7 27 CDS 1 +45 2 197594 AC079779.7 26 CDS 1 +46 2 197595 AC079779.7 26 CDS 1 +47 2 197596 AC079779.7 26 CDS 1 +48 2 197597 AC079779.7 25 CDS 1 +49 2 197598 AC079779.7 25 CDS 1 +52329 Y 2803782 ZFY 0 CDS 1 +52330 Y 2803783 ZFY 0 CDS 1 +52331 Y 2803784 ZFY 0 CDS 1 +52332 Y 2803785 ZFY 0 CDS 1 +52333 Y 2803786 ZFY 0 CDS 1 +52334 Y 2803787 ZFY 0 CDS 1 +52335 Y 2803788 ZFY 0 CDS 1 +52336 Y 2803789 ZFY 0 CDS 1 +52337 Y 2803790 ZFY 0 CDS 1 +52338 Y 2803791 ZFY 0 CDS 1 +52339 Y 2803792 ZFY 0 CDS 1 +52340 Y 2803793 ZFY 0 CDS 1 +52341 Y 2803794 ZFY 0 CDS 1 +52342 Y 2803795 ZFY 0 CDS 1 +52343 Y 2803796 ZFY 0 CDS 1 +52344 Y 2803797 ZFY 0 CDS 1 +52345 Y 2803798 ZFY 0 CDS 1 +52346 Y 2803799 ZFY 0 CDS 1 +52347 Y 2803800 ZFY 0 CDS 1 +52348 Y 2803801 ZFY 0 CDS 1 +52349 Y 2803802 ZFY 0 CDS 1 +52350 Y 2803803 ZFY 0 CDS 1 +52351 Y 2803804 ZFY 0 CDS 1 +52352 Y 2803805 ZFY 0 CDS 1 +52353 Y 2803806 ZFY 0 CDS 1 +52354 Y 2803807 ZFY 0 CDS 1 +52355 Y 2803808 ZFY 0 CDS 1 +52356 Y 2803809 ZFY 0 CDS 1 +52357 Y 2803810 ZFY 0 CDS 1 +52358 Y 2803811 ZFY 0 CDS 1 +52359 Y 2803812 ZFY 0 CDS 1 +52360 Y 2803813 ZFY 0 CDS 1 +52361 Y 2803814 ZFY 0 CDS 1 +52362 Y 2803815 ZFY 0 CDS 1 +52363 Y 2803816 ZFY 0 CDS 1 +52364 Y 2803817 ZFY 0 CDS 1 +52365 Y 2803818 ZFY 0 CDS 1 +52366 Y 2803819 ZFY 0 CDS 1 +52367 Y 2803820 ZFY 0 CDS 1 +52368 Y 2803821 ZFY 0 CDS 1 +52369 Y 2803822 ZFY 0 CDS 1 +52370 Y 2803823 ZFY 0 CDS 1 +52371 Y 2803824 ZFY 0 CDS 1 +52372 Y 2803825 ZFY 0 CDS 1 +52373 Y 2803826 ZFY 0 CDS 1 +52374 Y 2803827 ZFY 0 CDS 1 +52375 Y 2803828 ZFY 0 CDS 1 +52376 Y 2803829 ZFY 0 CDS 1 +52377 Y 2803830 ZFY 0 CDS 1 +52378 Y 2803831 ZFY 0 CDS 1 From 967e2d9dff751cd2f59ec9d3e4b5a127c87a5e0d Mon Sep 17 00:00:00 2001 From: RoanKanninga Date: Wed, 30 Aug 2023 17:17:39 +0200 Subject: [PATCH 8/8] update workflow dependency --- workflow_DRAGEN.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflow_DRAGEN.csv b/workflow_DRAGEN.csv index 75d26de0..bdcadb01 100644 --- a/workflow_DRAGEN.csv +++ b/workflow_DRAGEN.csv @@ -6,4 +6,4 @@ s03_AnnotateVcf,protocols/AnnotateVcf_perSample.sh,s01_PrepareDragenData;variant s04_SnpEff,protocols/SnpEff_perSample.sh,s03_AnnotateVcf s08_Gavin,protocols/Gavin.sh,s04_SnpEff;inputFile=sampleVariantCallsSnpEff_Annotated s09_ReheaderVcf,protocols/ReheaderVCF.sh,s08_Gavin -s10_PipelineFinished,protocols/PipelineFinished.sh,s01b_processDragenTrendAnalysis;s02_CoverageCalculations;s09_ReheaderVcf +s10_PipelineFinished,protocols/PipelineFinished.sh,s01b_ProcessDragenTrendAnalysis;s02_CoverageCalculations;s09_ReheaderVcf