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\noindent \begin{minipage}{\linewidth } \includegraphics[width=6cm]{frog}\par \textbf {Figure 2--Figure supplement 1.} There is no limit on the number of Figure Supplements for any one primary figure. Each figure supplement should be clearly labelled, Figure 1--Figure Supplement 1, Figure 1--Figure Supplement 2, Figure 2--Figure Supplement 1 and so on, and have a short title (and optional legend). Figure Supplements should be referred to in the legend of the associated primary figure, and should also be listed at the end of the article text file.\par \vskip 8pt \end{minipage} \vskip 16pt
\noindent \begin{minipage}{\linewidth } \includegraphics[width=6cm]{frog}\par \textbf {Figure 3--Figure supplement 1.} This is a supplementary figure's full caption, which will be used at the end of the manuscript.\par \vskip 8pt \end{minipage} \vskip 16pt
\noindent \begin{minipage}{\linewidth } \includegraphics[width=6cm]{frog}\par \textbf {Figure 3--Figure supplement 2.} This is another supplementary figure.\par \vskip 8pt \end{minipage} \vskip 16pt