diff --git a/tools/decontam/decontam.xml b/tools/decontam/decontam.xml index 352331d37bc..5a3eb92abd4 100644 --- a/tools/decontam/decontam.xml +++ b/tools/decontam/decontam.xml @@ -1,4 +1,5 @@ + Identification and removal of contaminants macros.xml @@ -150,6 +151,18 @@ control samples. Currently, only the negative control sample method is implemented in this wrapper. +**Method** + +To identify contaminants decontam requires a defined set of “negative control” samples in + which sequencing was performed on blanks without any biological sample added. +Extraction controls are preferred, and in amplicon sequencing the negative controls + should also be carried through the PCR step, as each step in the workflow + has the potential to introduce new contaminants. + +Decontam then uses Fisher’s exact tests to detect weather a feature is a contaminant or not, +by comparing true samples to ngeative controls. For more details on the detection method see +the publication of decontam. + **Output** - If a phyloseq object is provided as input, the output will be a phyloseq