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I have a question regarding the output of the tool varscan pileup2snp. The input is the pileup file generated by samtools mpileup without reference. One particular line from the input is
1 16259813 N 80 G$g$aaGAGAaggaAGGaaGaGAgaAGaAgAAGGaAAAAAGAAaaGAaGgGgAGaGAGAgGggGAAAAgAAGgggAGGGAA^]G BBCDigfDIHEHDgBJGDHDlkHcBJEJ]J/H?JiJmJIjJJFkJG^F?<JlFIJmJDJACJIEeJCJEJCDCHHHH>F@
So there are 80 reads covering this position, there is an N, since we don't have a reference. As I understand it, there are basically two nucleotides (G and A) found in this position. In the output there are these corresponding two lines
Chrom Position ... VarAllele 1 16259813 ... A 1 16259813 ... A
This is confusing to me, since I expect a G instead of the second A. Can anybody help me understand this issue? Thx.
The output file was generated by varscan pileup2snp pileup.tsv --min-coverage 10 --min-base-qual 30 --output-vcf 1 > output.txt
The text was updated successfully, but these errors were encountered:
I have a question regarding the output of the tool varscan pileup2snp. The input is the pileup file generated by samtools mpileup without reference. One particular line from the input is
1 16259813 N 80 G$g$aaGAGAaggaAGGaaGaGAgaAGaAgAAGGaAAAAAGAAaaGAaGgGgAGaGAGAgGggGAAAAgAAGgggAGGGAA^]G BBCDigfDIHEHDgBJGDHDlkHcBJEJ]J/H?JiJmJIjJJFkJG^F?<JlFIJmJDJACJIEeJCJEJCDCHHHH>F@
So there are 80 reads covering this position, there is an N, since we don't have a reference. As I understand it, there are basically two nucleotides (G and A) found in this position. In the output there are these corresponding two lines
Chrom Position ... VarAllele
1 16259813 ... A
1 16259813 ... A
This is confusing to me, since I expect a G instead of the second A. Can anybody help me understand this issue? Thx.
The output file was generated by
varscan pileup2snp pileup.tsv --min-coverage 10 --min-base-qual 30 --output-vcf 1 > output.txt
The text was updated successfully, but these errors were encountered: