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The rnaSpades mode is heavily focused on mRNA transcriptome assembly and is very aggressive at splicing assemblies into short transcripts. The —meta mode would be more suitable for our DNA/RNA assembly even though it is optimized for DNA metagenomes.
One barrier is that meta spades only accepts a single paired end library and doesn’t accept unpaired reads for now. Although not too common, we should handle this by warning after Trimmomatic if a significant number of reads become orphaned.
The text was updated successfully, but these errors were encountered:
The rnaSpades mode is heavily focused on mRNA transcriptome assembly and is very aggressive at splicing assemblies into short transcripts. The —meta mode would be more suitable for our DNA/RNA assembly even though it is optimized for DNA metagenomes.
One barrier is that meta spades only accepts a single paired end library and doesn’t accept unpaired reads for now. Although not too common, we should handle this by warning after Trimmomatic if a significant number of reads become orphaned.
The text was updated successfully, but these errors were encountered: