91.expanded_view_material.md

Expanded View

Expanded View Figures

{#fig:suppl_tomogram_slices width="15cm" tag="EV1"}

{#fig:suppl_segmentation width="15cm" tag="EV2"}

{#fig:suppl_histograms width="15cm" tag="EV3"}

{#fig:suppl_connected_slices width="8cm" tag="EV4"}

Expanded View Tables

ID	Exocytosis stage	Vesicles per tomogram	Tethers per AZ	AZ surface area [µm²]	Connectors per synapse (0-250 nm)
Control 1	not stimulated	220	15	0.11	331
Control 2	not stimulated	104	8	0.04	264
Control 3	not stimulated	127	4	0.05	230
Control 4	not stimulated	143	3	0.03	482
Control 5	not stimulated	213	12	0.11	361
Control 6	not stimulated	104	7	0.06	199
Control 7	not stimulated	184	9	0.03	360
Control 8	not stimulated	132	19	0.09	226
Control 9	not stimulated	134	6	0.08	326
Spray 1	late	697	5	0.03	272
Spray 2	late	115	3	0.08	88
Spray 3	late	429	21	0.19	882
Spray 5	early	534	57	0.07	1412
Spray 5_2 (second AZ, same synaptosome)	early		32	0.15
Spray 6	late	371	1	0.03	397
Spray 7	early	107	5	0.02	156
Spray 8	late	99	4	0.02	202
Spray 10	late	76	4	0.02	96
Table: Summary of the synaptosome tomograms.
{#tbl:synaptosome-tomograms tag="EV1"}

ID	Mutation	Vesicles per Tomogram	Tethers per AZ	AZ surface area [µm²]	Connectors per synapse (0-250 nm)
73	4E	0-459	23	0.23	269
80	4E	105	6	0.21	84
84	4E	109	10	0.12	107
88	4E	154	10	0.13	159
102	4E	103	0	0.29	94
114	4K	123	2	0.09	68
115	4K	137	1	0.26	70
116	4K	278	1	0.13	154
123	4K	55	3	0.09	52
128	WT-KO	243	12	0.18	683
132	WT-KO	126	2	0.24	110
133	WT-KO	505	27	0.17	229
134	WT-KO	600	7	0.34	144
Table: Summary of the neuron tomograms.
{#tbl:neuron-tomograms tag="EV2"}

Expanded View Movies

**Movie EV1: Tomogram with segmentation of synaptosome with late fusion events.** off-white = cell outline; pink = active zone; blue = synaptic vesicles; dark green = mitochondria; light green = large vesicles; yellow = connectors, red = tethers, scale bar 100nm

**Movie EV2: Tomogram with segmentation of WT SNAP-25 neurons.** off-white= cell outline; pink = active zone; blue = synaptic vesicles; green = large vesicles; yellow = connectors, red = tethers, scale bar 100nm