diff --git a/tests/data/b_p_w_vars.yaml b/tests/data/b_p_w_vars.yaml
index 8227b8a..bff4fa8 100644
--- a/tests/data/b_p_w_vars.yaml
+++ b/tests/data/b_p_w_vars.yaml
@@ -2,7 +2,7 @@
   vars:
     ansible_bender:
       verbose_layer_names: true
-      base_image: "docker.io/library/python:3-alpine"
+      base_image: "quay.io/biocontainers/python:3"
 
       working_container:
         volumes:
diff --git a/tests/data/p_w_vars_files.yaml b/tests/data/p_w_vars_files.yaml
index 95d5a51..95b2a6e 100644
--- a/tests/data/p_w_vars_files.yaml
+++ b/tests/data/p_w_vars_files.yaml
@@ -1,7 +1,7 @@
 - hosts: all
   vars:
     ansible_bender:
-      base_image: "docker.io/library/python:3-alpine"
+      base_image: "quay.io/biocontainers/python:3"
       target_image:
         name: with-vars-files
         labels:
diff --git a/tests/data/pb_wrong_type.yaml b/tests/data/pb_wrong_type.yaml
index 8381b8f..a39599e 100644
--- a/tests/data/pb_wrong_type.yaml
+++ b/tests/data/pb_wrong_type.yaml
@@ -1,7 +1,7 @@
 - hosts: all
   vars:
     ansible_bender:
-      base_image: "docker.io/library/python:3-alpine"
+      base_image: "quay.io/biocontainers/python:3"
       target_image: my-image-name
   tasks:
   - copy:
diff --git a/tests/data/playbook_with_unknown_keys.yaml b/tests/data/playbook_with_unknown_keys.yaml
index 47f650c..5f3402d 100644
--- a/tests/data/playbook_with_unknown_keys.yaml
+++ b/tests/data/playbook_with_unknown_keys.yaml
@@ -6,7 +6,7 @@
         name: unknown_key
 
       verbose_layer_names: true
-      base_image: "docker.io/library/python:3-alpine"
+      base_image: "quay.io/biocontainers/python:3"
 
       working_container:
         volumes:
diff --git a/tests/functional/test_cli.py b/tests/functional/test_cli.py
index e98ddf8..008e0aa 100644
--- a/tests/functional/test_cli.py
+++ b/tests/functional/test_cli.py
@@ -29,7 +29,7 @@ def test_inspect_cmd(tmpdir, target_image):
     ab(cmd, str(tmpdir))
     out = ab(["inspect"], str(tmpdir), return_output=True)
 
-    assert "base_image: docker.io/library/python:3-alpine" in out
+    assert "base_image: quay.io/biocontainers/python:3" in out
     assert "build_container: " in out
     assert "build_finished_time: " in out
     assert "build_start_time: " in out
diff --git a/tests/functional/test_conf.py b/tests/functional/test_conf.py
index bc29f7d..920d083 100644
--- a/tests/functional/test_conf.py
+++ b/tests/functional/test_conf.py
@@ -24,7 +24,7 @@ def test_basic(tmpdir):
         cmd = ["inspect", "--json"]
         ab_inspect_data = json.loads(ab(cmd, str(tmpdir), return_output=True))
 
-        assert ab_inspect_data["base_image"] == "docker.io/library/python:3-alpine"
+        assert ab_inspect_data["base_image"] == "quay.io/biocontainers/python:3"
         assert ab_inspect_data["build_id"] == "1"
         assert ab_inspect_data['build_volumes'] == [f'{data_dir}:/src:Z']
         assert ab_inspect_data['builder_name'] == "buildah"
@@ -65,7 +65,7 @@ def test_with_vars_files(tmpdir):
         cmd = ["inspect", "--json"]
         ab_inspect_data = json.loads(ab(cmd, str(tmpdir), return_output=True))
 
-        assert ab_inspect_data["base_image"] == "docker.io/library/python:3-alpine"
+        assert ab_inspect_data["base_image"] == "quay.io/biocontainers/python:3"
         assert ab_inspect_data["build_id"] == "1"
         assert ab_inspect_data['builder_name'] == "buildah"
         assert len(ab_inspect_data['layers']) == 3
diff --git a/tests/integration/test_api.py b/tests/integration/test_api.py
index 5e5e8c2..8769f87 100644
--- a/tests/integration/test_api.py
+++ b/tests/integration/test_api.py
@@ -303,7 +303,7 @@ def test_pb_with_role(build, application):
 
 @pytest.mark.parametrize("image_name,interpreter", [
     ("registry.fedoraproject.org/fedora:35", "/usr/bin/python3"),
-    ("docker.io/library/python:2.7-alpine", "/usr/local/bin/python2"),
+    ("quay.io/biocontainers/python:2.7", "/usr/local/bin/python2"),
     ("registry.access.redhat.com/ubi8/ubi:8.2", "/usr/libexec/platform-python")
 ])
 def test_cache_python_interpreter(application, build, image_name, interpreter):
diff --git a/tests/integration/test_buildah.py b/tests/integration/test_buildah.py
index 94c2c9d..efd3320 100644
--- a/tests/integration/test_buildah.py
+++ b/tests/integration/test_buildah.py
@@ -28,7 +28,7 @@
     ("registry.fedoraproject.org/fedora:33", True),
     ("registry.access.redhat.com/ubi8/python-38", True),
     (base_image, True),
-    ("docker.io/library/busybox", False),
+    ("quay.io/quay/busybox", False),
     ])
 def test_find_py_intrprtr_in_fedora_image(image_name, found):
     build = Build()
diff --git a/tests/integration/test_conf.py b/tests/integration/test_conf.py
index 0597f54..51e494c 100644
--- a/tests/integration/test_conf.py
+++ b/tests/integration/test_conf.py
@@ -17,7 +17,7 @@
 def test_expand_pb_vars():
     p = PbVarsParser(b_p_w_vars_path)
     data = p.expand_pb_vars()
-    assert data["base_image"] == "docker.io/library/python:3-alpine"
+    assert data["base_image"] == "quay.io/biocontainers/python:3"
     assert data["verbose_layer_names"]
     playbook_dir = os.path.dirname(b_p_w_vars_path)
     assert data["working_container"]["volumes"] == [f"{playbook_dir}:/src:Z"]
diff --git a/tests/spellbook.py b/tests/spellbook.py
index 9b609a1..252c0a6 100644
--- a/tests/spellbook.py
+++ b/tests/spellbook.py
@@ -26,7 +26,7 @@
 import_playbook_basic = os.path.join(data_dir, "import_playbook_basic.yaml")
 import_playbook_recursive = os.path.join(data_dir, "import_playbook_recursive.yaml")
 
-base_image = "docker.io/library/python:3-alpine"
+base_image = "quay.io/biocontainers/python:3"
 
 
 C7_AP_VER_OUT = """\