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176_common.txt
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Enzyme Target sequence Cutting position(+ -> -)
AanI TTATAA 4
AasI GACNNNNNNGTC 8
AatII GACGTC 6
Acc65I GGTACC 2
AdeI CACNNNGTG 7
AjuI* NNNNNNNNNNNNGAANNNNNNNTTGGNNNNNNNNNNN 6,38
AluI AGCT 3
Alw21I GWGCWC 6
Alw26I GTCTCNNNNN 7
Alw44I GTGCAC 2
ApaI GGGCCC 6
BamHI GGATCC 2
BclI TGATCA 2
BcnI CCSGG 3
BcuI ACTAGT 2
BfmI CTRYAG 2
BfoI RGCGCY 6
BglI GCCNNNNNGGC 8
BglII AGATCT 2
Bme1390I CCNGG 3
BmsI GCATCNNNNNNNNN 11
BoxI GACNNNNGTC 6
BpiI GAAGACNNNNNN 9
BplI* NNNNNNNNGAGNNNNNCTCNNNNNNNNNNNNN 1,33
Bpu10I CCTNAGCNNNNN 8
Bpu1102I GCTNAGC 3
BseDI CCNNGG 2
BseGI GGATGNN 8
BseJI GATNNNNATC 6
BseLI CCNNNNNNNGG 8
BseMI GCAATG 9
BseMII CTCAGNNNNNNNNNN 16
BseNI ACTGGN 7
BseSI GKGCMC 6
BseXI GCAGCNNNNNNNN 14
Bsh1236I CGCG 3
Bsh1285I CGRYCG 5
BshNI GGYRCC 2
BshTI ACCGGT 2
Bsp119I TTCGAA 3
Bsp120I GGGCCC 2
Bsp1407I TGTACA 2
Bsp143I GATC 1
BspLI GGNNCC 4
BspOI GCTAGC 6
BspTI CTTAAG 2
Bst1107I GTATAC 4
BstXI CCANNNNNNTGG 9
Bsu15I ATCGAT 3
BsuRI GGCC 3
BveI ACCTGCNNNN 11
CaiI CAGNNNCTG 7
Cfr10I RCCGGY 2
Cfr13I GGNCC 2
CpoI CGGWCCG 3
CseI GACGCNNNNN 11
CsiI ACCWGGT 2
Csp6I GTAC 2
DpnI GATC 3
DraI TTTAAA 4
Eam1104I CTCTTCN 8
Eam1105I GACNNNNNGTC 7
Ecl136II GAGCTC 4
Eco105I TACGTA 2
Eco130I CCWWGG 2
Eco147I AGGCCT 4
Eco31I GGTCTCN 8
Eco32I GATATC 4
Eco47I GGWCC 2
Eco47III AGCGCT 4
Eco52I CGGCCG 2
Eco57I CTGAAGNNNNNNNNNNNNNNNN 23
Eco72I CACGTG 4
Eco81I CCTNAGG 3
Eco88I CYCGRG 2
Eco91I GGTNACC 2
EcoO109I RGGNCCY 3
EcoRI GAATTC 2
EheI GGCGCC 4
Esp3I CGTCTCN 8
FaqI GGGACNNNNNNNNNN 16
FokI GGATGNNNNNNNNN 14
FspAI RTGCGCAY 5
FspBI CTAG 2
GsuI CTGGAGNNNNNNNNNNNNNNNN 23
HhaI GCGC 4
Hin1I GRCGYC 3
Hin1II CATG 5
Hin6I GCGC 2
HincII GTYRAC 4
HindIII AAGCTT 2
HinfI GANTC 2
HpaII CCGG 2
Hpy8I GTNNAC 4
HpyF10VI GCNNNNNNNGC 8
HpyF3I CTNAG 2
KflI GGGWCCC 3
KpnI GGTACC 6
Kpn2I TCCGGA 2
KspAI GTTAAC 4
LguI GCTCTTCN 9
Lsp1109I GCAGCNNNNNNNN 14
MauBI CGCGCGCG 3
MbiI CCGCTCNNN 7
MboI GATC 1
MboII GAAGANNNNNNNN 14
MlsI TGGCCA 4
MluI ACGCGT 2
MnlI CCTCNNNNNNN 12
Mph1103I ATGCAT 6
MreI CGCCGGCG 3
MspI CCGG 2
MssI GTTTAAAC 5
MunI CAATTG 2
Mva1269I GAATGCN 8
MvaI CCWGG 3
NcoI CCATGG 2
NdeI CATATG 3
NheI GCTAGC 2
NmuCI GTSAC 1
NotI GCGGCCGC 3
NsbI TGCGCA 4
OliI CACNNNNGTG 6
PacI TTAATTAA 6
PaeI GCATGC 6
PagI TCATGA 2
PdiI GCCGGC 4
PdmI GAANNNNTTC 6
PfeI GAWTC 2
Pfl23II CGTACG 2
PfoI TCCNGGA 2
Ppu21I YACGTR 4
Psp1406I AACGTT 3
Psp5II RGGWCCY 3
PspFI CCCAGCN 7
PstI CTGCAG 6
PsuI RGATCY 2
PsyI GACNNNGTC 5
PteI GCGCGC 2
PvuI CGATCG 5
PvuII CAGCTG 4
RruI TCGCGA 4
RsaI GTAC 3
RseI CAYNNNNRTG 6
SacI GAGCTC 6
SalI GTCGAC 2
SaqAI TTAA 2
SatI GCNGC 3
ScaI AGTACT 4
SchI GAGTCNNNNN 11
SdaI CCTGCAGG 7
SduI GDGCHC 6
SfaAI GCGATCGC 6
SfiI GGCCNNNNNGGCC 9
SgsI GGCGCGCC 3
SmaI CCCGGG 4
SmiI ATTTAAAT 5
SsiI CCGCNNN 5
SspI AATATT 4
TaaI ACNGT 4
TaiI ACGT 5
TaqI TCGA 2
TasI AATT 1
TatI WGTACW 2
TauI GCSGC 5
Tru1I TTAA 2
TscAI CASTGNN 8
Van91I CCANNNNNTGG 8
VspI ATTAAT 3
XagI CCTNNNNNAGG 6
XapI RAATTY 2
XbaI TCTAGA 2
XceI RCATGY 6
XhoI CTCGAG 2
XmaJI CCTAGG 2
XmiI GTMKAC 3