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config.json
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{
"bowtie2_rules": {
"platform": "Illumina",
"mapping_params": {
"default": ""
},
"load_env": "module load bioinfo-tools bowtie2/2.2.3 samtools/1.1"
},
"samtools_rules": {
"load_env": "module load bioinfo-tools samtools/1.1 BEDTools/2.21.0 picard/1.118",
"picard_jars": "/sw/apps/bioinfo/picard/1.118/milou"
},
"mapping_report_rules": {},
"concoct_rules": {
"load_env": "source activate concoct && module load bioinfo-tools MUMmer/3.23 # CONCOCT dev f4cb9fcb",
"mapper": "bowtie2",
"mapping_params": "default",
"concoct_params": {
"cutoff-1000-maxcluster-1000": "-c 1000 -l 1000"
},
"scripts_dir": "/home/alneberg/repos/CONCOCT/scripts"
},
"prodigal_rules": {
"load_env": "module load bioinfo-tools prodigal/2.60",
"prodigal_params": {
"default-meta": "-p meta"
}
},
"prokka_extended_rules": {
"dbs": ["cog", "pfam", "tigr"],
"prokka_name": "prokka_mod",
"load_env": "source activate concoct && module load bioinfo-tools BioPerl blast/2.2.28+ hmmer prodigal gnuparallel",
"cddid_all": "/proj/b2010008/nobackup/database/cddid_all.tbl",
"EXTEND": {
"gff": "toolbox/scripts/extended_prokka/extend_gff.py"
},
"get_prokka_annotation_script": "toolbox/scripts/extended_prokka/get.prokka.annotation.sh",
"collate_annotations_script": "toolbox/scripts/extended_prokka/collate.annotations.py",
"prokka_ops": "--metagenome",
"contigs": {},
"threads": "2"
},
"rpsblast_rules": {
"load_env": "module load bioinfo-tools blast/2.2.29+ gnuparallel/20140222",
"rpsblast_params": {
"default-concoct": "-outfmt '6 qseqid sseqid evalue pident score qstart qend sstart send length slen' -max_target_seqs 1 -evalue 0.00001"
},
"databases": {
"cog": "/sw/data/uppnex/cdd/little_endian/Cog"
},
"parallel_params": "-N 10 --env PATH"
},
"hmmer_rules": {
"load_env": "module load bioinfo-tools hmmer/3.1b1-gcc",
"hmmer_params": {
"default-concoct": "-E 0.0001 --cpu 16"
},
"databases": {
"all_pog": ""
}
},
"assembly_dir": ["data/contigs"],
"fastq_dirs": ["data/reads"],
"toolbox_dir": "toolbox"
}